Strain Data Sheet

RBRC11365

Information1

Image
BRC No.RBRC11365
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6;Cg-Gad1<tm1.1(tTA2)Kftnk>
Former Common nameGad1-tTA knock-in mice
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Yuchio Yanagawa, Gunma University, and Junichi Nakai, Tohoku University in 2006. Homozygous mice are neonatally lethal.
Strain descriptiontTA2 knockin mice at the Gad1 locus.
Colony maintenance
ReferencesMol. Brain, 14(1):5 (2021). 33413507
Cell Report 2, 397-406, 2012 22096083

Health Report

Examination Date / Room / Rack2023/06/13Room:3-5Rack:C
2023/05/22Room:3-5Rack:C

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Gad1glutamate decarboxylase 12Gad1<tm1.1(tTA2)Kftnk>targeted mutation 1.1, Kenji Tanaka

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP2loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
tTA2synthetic modified tetracycline-regulated transactivator: E. coli TetR (the repressor of tetracycline resistance operon), herpes simplex virus VP16 transcription avtivating domain (C-terminal portion)2tTA2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyA2

Information2

Donor DNAtTA2（synthetic modified tetracycline-regulated transactivator: E. coli TetR (the repressor of tetracycline resistance operon), herpes simplex virus VP16 transcription avtivating domain (C-terminal portion)(Urlinger et al., 2000)), SV40 polyA, phage P1 loxP site, mouse Gad1 genomic DNA
Research applicationCre/loxP system
Tet system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Mol. Brain, 14(1):5 (2021). The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of not a not-for profit institution for a not-for-profit research.
DepositorYuchio Yanagawa (Gunma University)
Strain Statusan icon for Live miceLive mice
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data