Strain Data Sheet

RBRC11364

Information1

Image
BRC No.RBRC11364
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6-Gad1<tm1(tetO)Ksak>
Former Common nameGad1-Stop-tetO knock-in mice
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Yuchio Yanagawa, Gunma University, and Kenji Sakimura, Niigata University in 2017. This strain was generated using RENKA ES cells derived from C57BL/6NCrlCrlj. Homozygous mice are neonatally lethal.
Strain descriptionFloxed stop cassette followed by tetO was knockined between the promoter and coding sequence of the Gad1.
Colony maintenance
ReferencesMol. Brain, 14(1):5 (2021). 33413507

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Gad1glutamate decarboxylase 12Gad1<tm1(tetO)Ksak>targeted mutation 1, Kenji Sakimura

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site2Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
HIS3His3 (S. cerevisiae)2HIS3

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP2loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)2E. coli EM7 promoter, Mouse Phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
tetOtetracycline operator (E. coli)2tetO

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyA2

Information2

Donor DNAP1 ファージ loxP, SV40ウイルス polyA, 酵母 frt, 大腸菌 EM7プロモーター, 大腸菌Neo(neomycin耐性遺伝子), 酵母 HIS3ターミネーター, 大腸菌 tetO配列, マウス Pgk promoter
Research applicationCre/loxP system
FLP/frt system
Tet system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Mol. Brain. 2021 Jan 7;14(1):5.The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of not a not-for profit institution for a not-for-profit research.
DepositorYuchio Yanagawa (Gunma University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data