Strain Data Sheet

RBRC11364

Strain Information

Image
BRC No.RBRC11364
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6-Gad1<tm1(tetO)Ksak>
Former Common nameGad1-Stop-tetO knock-in mice
H-2 Haplotype
ES Cell lineRENKA [C57BL/6NCrlCrlj]
Background strain
Appearance
Strain developmentDeveloped by Yuchio Yanagawa, Gunma University, and Kenji Sakimura, Niigata University in 2017. This strain was generated using RENKA ES cells derived from C57BL/6NCrlCrlj. Homozygous mice are neonatally lethal.
Strain descriptionFloxed stop cassette followed by tetO was knockined between the promoter and coding sequence of the Gad1.
Colony maintenance
References
Global knockdown of glutamate decarboxylase 67 elicits emotional abnormality in mice.
Miyata S, Kakizaki T, Fujihara K, Obinata H, Hirano T, Nakai J, Tanaka M, Itohara S, Watanabe M, Tanaka K F, Abe M, Sakimura K, Yanagawa Y
Mol. Brain, 14(1):5 (2021). 33413507

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frt yeast FRT (flippase recombination target) site2Frt
Gad1
MGI:95632		glutamate decarboxylase 12Gad1<tm1(tetO)Ksak> targeted mutation 1, Kenji Sakimura
  • developmental and epileptic encephalopathy 89(MedGEN)
    • HIS3 His3 (S. cerevisiae)2HIS3
    loxP phage P1 loxP2loxP
    neo neomycin resistance gene (E. coli)2 E. coli EM7 promoter, Mouse Phosphoglycerate kinase promoter (PGK promoter)
    tetO tetracycline operator (E. coli)2tetO
    SV40 polyA2

    Phenotype

    Phenotype annotation from literatures by Mammalian phenotype ontology
    Detailed phenotype data

    Ordering Information

    Donor DNAP1 ファージ loxP, SV40ウイルス polyA, 酵母 frt, synthetic bacterial EM7 promoter (T7 phage), 大腸菌Neo（neomycin耐性遺伝子）, 酵母 HIS3ターミネーター, 大腸菌 tetO配列, マウス Pgk promoter
    Research applicationCre/loxP system
    FLP/frt system
    Tet system
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Mol. Brain. 2021 Jan 7;14(1):5.The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of not a not-for profit institution for a not-for-profit research.
    DepositorYuchio Yanagawa (Gunma University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovery and QC required prior to distribution
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    BRC mice in Publications

    No Data