Strain Data Sheet

RBRC11357

Information1

Image
BRC No.RBRC11357
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameB6.B6D2-H2ax<em1Ibbr>
Former Common nameH2A.X<+/->
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Dai Tsukioka and Fugaku Aoki, The University of Tokyo, in 2021. Mice were generated using fertilized eggs derived from BDF1 with the CRISPR/Cas9. Mice were backcrossed to C57BL/6J for six generation. C57BL/6 congenic strain. Homozygous males are infertile, whereas homozygous females deliver smaller number of pups.
Strain descriptionH2ax mutant mouse generated by the CRISPR/Cas9. 38 bp deletion in the H2ax gene.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
References

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
H2axH2A.X variant histone9H2ax<em1Ibbr>endonuclease-mediated mutation 1, Fugaku Aoki

Information2

Donor DNA
Research application
Specific Term and ConditionsNo specific terms and conditions. (The DEPOSITOR waives its own rights under any patents, intellectual property, or other proprietary rights with respect to the results to be obtained by use of the BIOLOGICAL RESOURCE.)
DepositorFugaku Aoki (The University of Tokyo)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data