Strain Data Sheet

RBRC11355

Information1

Image
BRC No.RBRC11355
TypeCRISPR/Cas9 (Transgene)Cartagena
SpeciesMus musculus
Strain nameB6.Cg-Clec7a<tm1Yiw> Card9<em1Yiw>
Former Common nameDectin-1/CARD9 dKO/B6
H-2 Haplotype
ES Cell lineE14.1 [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeposited by Yoichiro Iwakura, Tokyo University of Science, in 2022. Mice were generated using fertilized eggs derived from RBRC05094 B6;129P2-Clec7a<tm1Yiw> with the CRISPR/Cas9. C57BL/6J congenic strain.
Strain descriptionClec7a/Card9 double mutant mice. 17bp deletion at the Clec7a gene. Clec7a<tm1Yiw> is derived from RBRC05094 B6;129P2-Clec7a<tm1Yiw>.
Colony maintenance
ReferencesNat. Immunol., 8, 39-46 (2007). 17159982

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Card9caspase recruitment domain family, member 92Card9<em1Yiw>endonuclease-mediated mutation 1, Yoichiro Iwakura

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Clec7aC-type lectin domain family 7, member a6Clec7a<tm1Yiw>targeted mutation 1, Yoichiro Iwakura

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)6neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAP1 Phage loxP site, mouse Phosphoglycerate kinase (PGK) promoter, E.coli Neomycin resistant gene, Bovine growth hormone polyadenylation site, mouse Clec7a genomic DNA
Research applicationCre/loxP system
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
DepositorYoichiro Iwakura (Tokyo University of Science)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data