Strain Data Sheet

RBRC11346

Information1

Image
BRC No.RBRC11346
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Actb<tm1.1(tetO-GFP/bPAC)Yksk>
Former Common nametetO-GFP-2A-bPAC
H-2 Haplotype
ES Cell lineG0G1
Background strain
Appearance
Strain developmentDeposited by Ryuta Koyama, The University of Tokyo in 2021.
Strain descriptiontetO-GFP-2A-bPAC
Colony maintenance
ReferencesProc. Natl. Acad. Sci. USA, 118(3) e2016584118 (2021). doi: 10.1073/pnas.2016584118. 33452135
Cell Rep., 36(4):109427(2021). 34320360

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Actbactin, beta5Actb<tm1.1(tetO-GFP/bPAC)Yksk>

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)5EGFPE. coli tetO (tetracycline operator), CMV promoter

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site5Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
T2AThosea asigna virus 2A peptide (insect)5T2A

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP5loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP5loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP5loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP5loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
photoactivated adenylyl cyclase (bPAC) Beggiatoa sp.5

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyA5

Information2

Donor DNAE. coli tetR(tetracycline repressor), E. coli tetO (tetracycline operator), CMV promoter, rabbit Beta globin intron, jellyfish EGFP cDNA, Thosea asigna virus 2A peptide (insect), Beggiatoa sp. photoactivated adenylyl cyclase (bPAC), SV40 polyA signal, Phage P1 loxP sites, Yeast FRT site, mouse Actb genomic DNA
Research applicationCre/loxP system
FLP/frt system
Fluorescent Proteins/lacZ System
Tet system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc Natl Acad Sci USA. Jan 19;118(3) (2021) :e2016584118. doi: 10.1073/pnas.2016584118.The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a research. The RECIPIENT must contact the DEPOSITOR in the case of a application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorRyuta Koyama (The University of Tokyo)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)
  4. GFP Transfer License (Japanese / English)
    Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.

Genotyping protocol -PCR-

BRC mice in Publications

No Data