Strain Information | |
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Image | |
BRC No. | RBRC11332 |
Type | CRISPR/Cas9 |
Species | Mus musculus |
Strain name | JF1/Ms-Ngly1<em1Ta> |
Former Common name | JF1-Ngly1 KO |
H-2 Haplotype | |
ES Cell line | |
Background strain | |
Appearance | |
Strain development | Deposided by Ryuichi Tozawa, Takeda Chemical Industries, Ltd. in 2021. |
Strain description | Ngly1 mutant mice in JF1/Ms genetic background. Whole coding region was deleted by the CRISPR/Cas9. |
Colony maintenance | |
References | JF1/B6F1 Ngly1(-/-) mouse as an isogenic animal model of NGLY1 deficiency. Asahina M, Fujinawa R, Fujihira H, Masahara-Negishi Y, Andou T, Tozawa R, Suzuki T Proc. Jpn. Acad. Ser. B Phys. Biol. Sci., 97(2):89-102 (2021). 33563880 |
Health Report | |
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Examination Date / Room / Rack | 2024/04/22Room:3-CRack:L 2024/01/22Room:3-CRack:L 2023/10/23Room:3-CRack:L 2023/07/24Room:3-CRack:L 2023/04/24Room:3-CRack:L 2023/01/23Room:3-CRack:L |
Gene | |||||||
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Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
Ngly1 | N-glycanase 1 | 14 | Ngly1 | endonuclease mediated mutation 1, |
Phenotype | |
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Annotation by Mammalian phenotyhpe ontology | |
Detailed phenotype data |
Ordering Information | |
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Donor DNA | mouse gRNA (guide RNA), mouse ssODN (single strand ODN) |
Research application | Mouse Models for Human Disease Neurobiology Research |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Jpn. Acad. Ser. B Phys. Biol. Sci., 97(2):89-102 (2021).The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. |
Depositor | Ryuichi Tozawa (Takeda Pharmaceutical Company Limited.) |
Strain Status | Live mice Frozen embryos Frozen sperm |
Strain Availability | Cryopreserved embryos (within 1 month) Live mouse (1 to 3 months) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |