Strain Data Sheet

RBRC11332

Information1

Image
BRC No.RBRC11332
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameJF1/Ms-Ngly1<em1Ta>
Former Common nameJF1-Ngly1 KO
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposided by Ryuichi Tozawa, Takeda Chemical Industries, Ltd. in 2021.
Strain descriptionNgly1 mutant mice in JF1/Ms genetic background. Whole coding region was deleted by the CRISPR/Cas9.
Colony maintenance
ReferencesProc. Jpn. Acad. Ser. B Phys. Biol. Sci., 97(2):89-102 (2021). 33563880

Health Report

Examination Date / Room / Rack2023/10/23Room:3-CRack:L
2023/07/24Room:3-CRack:L
2023/04/24Room:3-CRack:L
2023/01/23Room:3-CRack:L
2022/10/25Room:3-CRack:L
2022/07/25Room:3-CRack:L
2022/04/25Room:3-CRack:L
2022/02/14Room:3-CRack:L

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Ngly1N-glycanase 114Ngly1<em1Ta>

Information2

Donor DNAmouse gRNA (guide RNA), mouse ssODN (single strand ODN)
Research applicationMouse Models for Human Disease
Neurobiology Research
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Jpn. Acad. Ser. B Phys. Biol. Sci., 97(2):89-102 (2021).The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorRyuichi Tozawa (Takeda Pharmaceutical Company Limited.)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Live mouse (1 to 3 months)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data