Strain Data Sheet

RBRC11332

Strain Information

Image
BRC No.RBRC11332
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameJF1/Ms-Ngly1<em1Ta>
Former Common nameJF1-Ngly1 KO
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposided by Ryuichi Tozawa, Takeda Chemical Industries, Ltd. in 2021.
Strain descriptionNgly1 mutant mice in JF1/Ms genetic background. Whole coding region was deleted by the CRISPR/Cas9.
Colony maintenance
References
JF1/B6F1 Ngly1(-/-) mouse as an isogenic animal model of NGLY1 deficiency.
Asahina M, Fujinawa R, Fujihira H, Masahara-Negishi Y, Andou T, Tozawa R, Suzuki T
Proc. Jpn. Acad. Ser. B Phys. Biol. Sci., 97(2):89-102 (2021). 33563880

Health Report

Examination Date / Room / Rack2025/11/17Room:3-CRack:LSentinel mouse program
2025/09/08Room:3-CRack:LSentinel mouse program
2025/06/30Room:3-CRack:LSentinel mouse program
2025/04/21Room:3-CRack:LSentinel mouse program
2025/01/20Room:3-CRack:LSentinel mouse program
2024/10/21Room:3-CRack:LSentinel mouse program
2024/07/22Room:3-CRack:LSentinel mouse program
2024/04/22Room:3-CRack:LSentinel mouse program
2024/01/22Room:3-CRack:LSentinel mouse program

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Ngly1
MGI:1913276		N-glycanase 114Ngly1<em1Ta> endonuclease mediated mutation 1,
  • obsolete NGLY1-deficiency(MedGEN)

    • Phenotype

    Phenotype annotation from literatures by Mammalian phenotype ontology
    Detailed phenotype data

    Ordering Information

    Donor DNAmouse gRNA (guide RNA), mouse ssODN (single strand ODN)
    Research applicationMouse Models for Human Disease
    Neurobiology Research
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Jpn. Acad. Ser. B Phys. Biol. Sci., 97(2):89-102 (2021).The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorRyuichi Tozawa (Takeda Pharmaceutical Company Limited.)
    Strain Statusan icon for Live miceLive mice
    an icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityCryopreserved embryos (within 1 month)
    Live mouse (1 to 3 months)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data