Strain Data Sheet

RBRC11326

Information1

Image
BRC No.RBRC11326
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameBlimp1-floxed mice, B6.Cg-Prdm1<tm2Masu>/Dkit
Former Common nameBlimp1fl, Blimp1 loxP, Blimp1(Prdm1)-floxed mouse
H-2 Haplotype
ES Cell lineE14.1 [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeposited by Alexander Tarakhovsky, Rockefeller University in 2021. This strain was generated using E14.1 ES cells derived from 129P2/OlaHsd and then backcrossed to C57BL/6N.
Strain descriptionFloxed Blimp1 (Prdm1) mouse. A pair of loxP flanks exon 5. A neo cassette was removed in this strain.
Colony maintenance
ReferencesNature, 436 (7048) 207-13 (2005). 15937476

Health Report

Examination Date / Room / Racknull

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Prdm1PR domain containing 1, with ZNF domain10Prdm1<tm2Masu>targeted mutation 2, M Azim SuraniBlimp1

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP10loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP10loxP

Information2

Donor DNAP1 phage loxP sites, Mouse Prdm1 genomic DNA
Research applicationCre/loxP system
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to the RECIPIENT at a not-for-profit institution for academic, non-commercial research. If the RECIPIENT of the BIOLOGICAL RESOURCE desires to use or license the BIOLOGICAL RESOURCE for COMMERCIAL PURPOSES(as defined below), the RECIPIENT is required in advance of such use, to negotiate in good faith with the DEPOSITOR to establish the terms of a commercial license. "COMMERCIAL PURPOSES" means the sale, lease license, or other transfer of the BIOLOGICAL RESOURCE to a for-profit organization. COMMERCIAL PURPOSES shall also include uses of the BIOLOGICAL RESOURCE by any or organization, including the RIKEN BRC, to perform contract research, to screen compound libraries, to produce or manufacture products for general sale, or to conduct research activities that result in any sale, lease license, or transfer of the BIOLOGICAL RESOURCE to a for-profit organization.
DepositorAlexander Tarakhovsky (The Rockefeller University)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data