Strain Data Sheet

RBRC11286

Strain Information

Image
BRC No.RBRC11286
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameC57BL/6J-Lta4h<em1Tyoko>
Former Common nameLTA4H-KO, Leukotriene A4 hydrolase KO mouse
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Takehiko Yokomizo, Juntendo University in 2021. C57BL/6J genetic background.
Strain descriptionLta4h mutant mice generated by the CRISPR/Cas9.
Colony maintenance
References
Expression of leukotriene B4 receptor 1 defines functionally distinct DCs that control allergic skin inflammation.
Tomoaki Koga, Fumiyuki Sasaki, Kazuko Saeki, Soken Tsuchiya, Toshiaki Okuno, Mai Ohba, Takako Ichiki, Satoshi Iwamoto, Hirotsugu Uzawa, Keiko Kitajima, Chikara Meno, Eri Nakamura, Norihiro Tada, Yoshinori Fukui, Junichi Kikuta, Masaru Ishii, Yukihiko Sugimoto, Mitsuyoshi Nakao, Takehiko Yokomizo
Cell Mol. Immunol., 18(6):1437-1449.(2020). 33037399

Leukotriene A4 hydrolase deficiency protects mice from diet-induced obesity by increasing energy expenditure through neuroendocrine axis.
Hirotsugu Uzawa, Daisuke Kohno, Tomoaki Koga, Tsutomu Sasaki, Ayako Fukunaka, Toshiaki Okuno, Airi Jo-Watanabe, Saiko Kazuno, Takeshi Miyatsuka, Tadahiro Kitamura, Yoshio Fujitani, Hirotaka Watada, Kazuko Saeki, Takehiko Yokomizo
FASEB J., 34(10):13949-13958 (2020). 32844470

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Lta4h
MGI:96836		leukotriene A4 hydrolase10Lta4h<em1Tyoko> endonuclease-mediated mutation 1, Takehiko Yokomizo

Phenotype

Phenotype annotation from literatures by Mammalian phenotype ontology
  • abnormal brown adipose tissue thermogenesis (MP:0011699)

  • abnormal catecholamine level (MP:0011479)

  • decreased susceptibility to chemically induced skin inflammation (MP:0012573)

  • decreased susceptibility to diet-induced obesity (MP:0005659)

  • increased circulating levels of thyroid hormone (MP:0003350)
    • more 2 phenotypes
  • increased circulating thyroid-stimulating hormone level (MP:0005122)

  • increased energy expenditure (MP:0004889)
    • Detailed phenotype data

    Ordering Information

    Donor DNA
    Research applicationImmunology and Inflammation Research
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Cell Mol. Immunol., 18(6):1437-1449.(2020). FASEB J 34, 13949-13958 (2020). The RECIPIENT must contact the DEPOSITOR in the case of a application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorTakehiko Yokomizo (Juntendo University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data