Strain Data Sheet

RBRC11281

Strain Information

Image
BRC No.RBRC11281
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6D2-Tg(HTT<*>/EGFP)190Nn
Former Common nameHD190QG
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Nobuyuki Nukina, RIKEN in 2021. B6D2F1 mixed genetic background. Natural mating and breeding are not practical. Reproductive technique (IVF) is necessary to maintain this strain. It is important to use mouse that has been confirmed to have the expected repeat length by PCR.
Strain descriptionTransgenic mice harboring human HTT promoter and exon1 with CAG repeats (about 190 repeats) fused with EGFP. Most mice die by 25 weeks.
Colony maintenance
References
Decreased expression of hypothalamic neuropeptides in Huntington disease transgenic mice with expanded polyglutamine-EGFP fluorescent aggregates.
Svetlana Kotliarova, Nihar R Jana, Naoaki Sakamoto, Masaru Kurosawa, Haruko Miyazaki, Munenori Nekooki, Hiroshi Doi, Yoko Machida, Hon Kit Wong, Taishi Suzuki, Chiharu Uchikawa, Yuri Kotliarov, Kazuyo Uchida, Yoshiro Nagao, Utako Nagaoka, Akira Tamaoka, Kiyomitsu Oyanagi, Fumitaka Oyama, Nobuyuki Nukina
J. Neurochem., 93(3):641-53 (2005). 15836623

Depletion of p62 reduces nuclear inclusions and paradoxically ameliorates disease phenotypes in Huntington's model mice.
Masaru Kurosawa, Gen Matsumoto, Yoshihiro Kino, Misako Okuno, Mizuki Kurosawa-Yamada, Chika Washizu, Harumi Taniguchi, Kazuhiro Nakaso, Toru Yanagawa, Eiji Warabi, Tomomi Shimogori, Takashi Sakurai, Nobutaka Hattori, Nobuyuki Nukina
Hum. Mol. Genet., 24(4):1092-105 (2015). 25305080

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
EGFP Enhanced Green Fluorescent Protein (Aequorea victoria)UNEGFP
SV40 polyAUN

Phenotype

Phenotype annotation from literatures by Mammalian phenotype ontology
  • abnormal involuntary movement (MP:0003492)

  • abnormal nervous system morphology (MP:0003632)

  • ataxia (MP:0001393)

  • limb grasping (MP:0001513)

  • premature death (MP:0002083)
    • more 1 phenotypes
  • tremors (MP:0000745)
    • Detailed phenotype data

    Ordering Information

    Donor DNAhuman HTT promoter, human Huntingtin (HTT) DNA, jellyfish EGFP cDNA, SV40 polyA
    Research applicationFluorescent Proteins/lacZ System
    Mouse Models for Human Disease
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J Neurochem. 2005 May;93(3):641-53. doi: 10.1111/j.1471-4159.2005.03035.x.
    DepositorNobuyuki Nukina (RIKEN CBS)
    Strain Statusan icon for Frozen embryosFrozen embryos
    Strain AvailabilityRecovery and QC required prior to distribution
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    BRC mice in Publications

    No Data