Strain Data Sheet

RBRC11278

Information1

Image
BRC No.RBRC11278
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6N-Pkn2<tm1.1Hmuk>
Former Common namePKN2<flox/flox>
H-2 Haplotype
ES Cell lineRENKA [C57BL/6NCrlCrlj]
Background strain
Appearance
Strain developmentestablished in 2009. back-crossed to C57BL/6N (Charles River) more than 10 times.
Strain descriptionExons 18 and 19 of PKN2, encoding the area around the activation loop of the catalytic domain of this enzyme, are flanked by two loxP sequences in the same orientation.
Colony maintenance
ReferencesGenes Cells, 22(2):220-236 (2017). doi: 10.1111/gtc.12470. Epub 2017 Jan 19. 28102564

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Pkn2protein kinase N23Pkn2<tm1.1Hmuk>targeted mutation 1.1, Hideyuki Mukai

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site3Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP3loxP

Information2

Donor DNAphage P1 loxP sites, yeast FRT (flippase recombination target) site, mouse Pkn2 genomic DNA
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genes Cells. 2017 Feb;22(2):220-236. doi: 10.1111/gtc.12470. Epub 2017 Jan 19.Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using e-mail.
DepositorHideyuki Mukai (Kobe University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data