Strain Data Sheet
RBRC11278
Strain Information | |
|---|---|
| Image | |
| BRC No. | RBRC11278 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | C57BL/6N-Pkn2<tm1.1Hmuk> |
| Former Common name | PKN2<flox/flox> |
| H-2 Haplotype | |
| ES Cell line | RENKA [C57BL/6NCrlCrlj] |
| Background strain | |
| Appearance | |
| Strain development | established in 2009. back-crossed to C57BL/6N (Charles River) more than 10 times. |
| Strain description | Exons 18 and 19 of PKN2, encoding the area around the activation loop of the catalytic domain of this enzyme, are flanked by two loxP sequences in the same orientation. |
| Colony maintenance | |
| References | PKN2 is essential for mouse embryonic development and proliferation of mouse fibroblasts. Danno S, Kubouchi K, Mehruba M, Abe M, Natsume R, Sakimura K, Eguchi S, Oka M, Hirashima M, Yasuda H, Mukai H Genes Cells, 22(2):220-236 (2017). doi: 10.1111/gtc.12470. Epub 2017 Jan 19. 28102564 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |||||||
|---|---|---|---|---|---|---|---|
| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| Frt | yeast FRT (flippase recombination target) site | 3 | Frt | ||||
| Pkn2 | protein kinase N2 | 3 | Pkn2 | targeted mutation 1.1, Hideyuki Mukai | |||
| loxP | phage P1 loxP | 3 | loxP | ||||
Phenotype | |
|---|---|
| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
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| Donor DNA | phage P1 loxP sites, yeast FRT (flippase recombination target) site, mouse Pkn2 genomic DNA |
| Research application | Cre/loxP system FLP/frt system |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genes Cells. 2017 Feb;22(2):220-236. doi: 10.1111/gtc.12470. Epub 2017 Jan 19.Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using e-mail. |
| Depositor | Hideyuki Mukai (Kobe University) |
| Strain Status |  Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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| No Data |