Strain Data Sheet



BRC No.RBRC11277
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6N-Pkn1<tm1.1(T778A)Hmuk>
Former Common namePKN1[T778A]
H-2 Haplotype
ES Cell lineRENKA [C57BL/6NCrlCrlj]
Background strain
Strain developmentestablished in 2007. back-crossed to Charles River C57BL/6N more than 10 times. homozygous mutant mice -- leukopenia, splenomegaly
Strain descriptionkinase-negative mutant of PKN1
Colony maintenance
ReferencesSci. Rep., 7(1):7663 (2017). doi: 10.1038/s41598-017-07936-9. 28794483

Health Report

Examination Date / Room / Rack


Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Pkn1protein kinase N18Pkn1<tm1.1(T778A)Hmuk>targeted mutation 1.1, Hideyuki Mukai

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP8loxP


Donor DNAphage P1 loxP site, mouse Pkn1 genomic DNA
Research applicationCre/loxP system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Sci Rep. Aug 9;7(1):7663 (2017). doi: 10.1038/s41598-017-07936-9.Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using e-mail.
DepositorHideyuki Mukai (Kobe University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data