Strain Data Sheet

RBRC11273

Strain Information

Image
BRC No.RBRC11273
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Mapk14<tm1(D316N)Moga>
Former Common namep38alpha floxSem
H-2 Haplotype
ES Cell lineEB3/5 [129/Ola]
Background strain
Appearance
Strain developmentEB3/5 ES cells derived from E14tg2aThe homo mice were embryonic lethal. After removing the gene cassette by the cre recombinase, the mice expressed p38alpha Mapk with D316N sevenmaker type mutation and home mice were viable.
Strain descriptionThe p38alpha (Mapk 14) gene is disrupted by by the insertion of an IRES-β-geo fusion cassette flanked by loxP sites. Homo mice are embryonic lethal.If the cassette is removed by cre mediated recombination, this mouse can conditionally express p38alpha with D316N sevenmaker type mutation (a docking site mutation).A 3.5-kb cassette flanked by two loxP sites is inserted between exons 10 and 11 of p38alpha gene. At the same time exon 11 is replaced by one coding the D316N sevenmaker type mutation. The cassette is generated by modifying the dicistronic targeting construct, pGT1.8IresBgeo (Mountford et al., 1994), consisting of a splice acceptor site (SA) from mouse En-2, an internal ribosomal entry site (IRES) from encephalomyocarditis virus (EMCV), a neomycin phosphotransferase gene (neo), and the SV40 polyadenylation sequence.
Colony maintenance
References
Complementation of placental defects and embryonic lethality by trophoblast-specific lentiviral gene transfer.
Okada Y, Ueshin Y, Isotani A, Saito-Fujita T, Nakashima H, Kimura K, Mizoguchi A, Oh-Hora M, Mori Y, Ogata M, Oshima R G, Okabe M, Ikawa M
Nat. Biotechnol., 25(2):233-7 (2007). 17220877

Critical role of p38 MAPK for regeneration of the sciatic nerve following crush injury in vivo.
Kato N, Matsumoto M, Kogawa M, Atkins G J, Findlay D M, Fujikawa T, Oda H, Ogata M
J. Neuroinflammation, 10:1. (2013). 23282009

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
SV40 polyA adenylation17
  • Noonan syndrome 13(MedGEN)
    • En2En2 SA (mouse En2 intron 2/exon 3 splice acceptor sequence)17En2
  • Noonan syndrome 13(MedGEN)
    • Iresinternal ribosomal entry site (EMCV)17
  • Noonan syndrome 13(MedGEN)
    • Mapk14mitogen-activated protein kinase 1417Mapk14targeted mutation 1, Masato Ogata
  • Noonan syndrome 13(MedGEN)
    • loxPphage P1 loxP17loxP
  • Noonan syndrome 13(MedGEN)
    • neoneomycin resistance gene (E. coli)17
  • Noonan syndrome 13(MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
    Detailed phenotype data

    Ordering Information

    Donor DNABacteriophage P1 loxP sites, mouse En-2 splice acceptor site, EMCV IRES, E. coli neo, SV40 Poly adenylation sequence, Mouse Mapk14 genomic DNA (exon 11)
    Research applicationCre/loxP system
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nat. Biotechnol., 25(2):233-7 (2007). J. Neuroinflammation, 10:1. (2013).
    DepositorMasato Ogata (Mie University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data