Strain Data Sheet

RBRC11272

Strain Information

Image
BRC No.RBRC11272
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Mapk1<tm3(D319N)Moga>
Former Common nameERK2 floxSem
H-2 Haplotype
ES Cell lineEB3/5 [129/Ola]
Background strain
Appearance
Strain developmentEB3/5 ES cells derived from E14tg2aThe homo mice were embryonic lethal. After removing the gene cassette by the cre recombinase, the mice expressed ERK2 Mapk with D319N sevenmaker type mutation.
Strain descriptionThe ERK2 (Mapk 1) gene of ERK2 floxSem mouse is disrupted by by the insertion of an IRES-β-geo fusion cassette flanked by loxP sites. Homo mice are embryonic lethal.If the cassette is removed by cre mediated recombination, this mouse can conditionally express ERK2 with D319N sevenmaker type mutation (a docking site mutation).A 3.5-kb cassette flanked by two loxP sites is inserted after exons 2 of ERK2 gene. At the same time exon 4 is replaced by a partial cDNA of ERK2 starting from exon 3 to the end of the polyadenylation signal sequence. This cDNA codes the D319N sevenmaker type mutation. The cassette is generated by modifying the dicistronic targeting construct, pGT1.8IresBgeo (Mountford et al., 1994), consisting of a splice acceptor site (SA) from mouse En-2, an internal ribosomal entry site (IRES) from encephalomyocarditis virus (EMCV), a neomycin phosphotransferase gene (neo), and the SV40 polyadenylation sequence.
Colony maintenance
References
Essential role for ERK2 mitogen-activated protein kinase in placental development.
Hatano N, Mori Y, Oh-hora M, Kosugi A, Fujikawa T, Nakai N, Niwa H, Miyazaki J, Hamaoka T, Ogata M
Genes Cells. 8(11):847-56 (2003). 14622137

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
SV40 polyA adenylation16
  • Noonan syndrome 13(MedGEN)
    • En2En2 SA (mouse En2 intron 2/exon 3 splice acceptor sequence)16En2
  • Noonan syndrome 13(MedGEN)
    • Iresinternal ribosomal entry site (EMCV)16
  • Noonan syndrome 13(MedGEN)
    • Mapk1mitogen-activated protein kinase 116Mapk1targeted mutation 3, Masato Ogata
  • Noonan syndrome 13(MedGEN)
    • loxPphage P1 loxP16loxP
  • Noonan syndrome 13(MedGEN)
    • neoneomycin resistance gene (E. coli)16
  • Noonan syndrome 13(MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
    Detailed phenotype data

    Ordering Information

    Donor DNABacteriophage P1 loxP sites, mouse En-2 splice acceptor site, EMCV IRES, E. coli neo, SV40 Poly adenylation sequence, mouse Mapk1 cDNA, pA, mouse Mapk1 genomic DNA
    Research applicationCre/loxP system
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genes Cells. 8(11):847-56 (2003).
    DepositorMasato Ogata (Mie University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data