Strain Data Sheet
RBRC11264
Strain Information | |
|---|---|
| Image | |
| BRC No. | RBRC11264 |
| Type | CRISPR/Cas9 |
| Species | Mus musculus |
| Strain name | B6D2-Rasef<em1Osb> |
| Former Common name | Rasef<-63153/wt> |
| H-2 Haplotype | |
| ES Cell line | |
| Background strain | |
| Appearance | |
| Strain development | Deposited by Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2020. Fertilized eggs derived from BDF1 × BDF1 were used to generate this line. Mixed genetic background. |
| Strain description | Rasef knockout mouse generated by the CRISPR/Cas9. 63,153 bp deletion in the Rasef gene. |
| Colony maintenance | |
| References | CRISPR/Cas9-mediated genome editing reveals 12 testis-enriched genes dispensable for male fertility in mice. Oyama Y, Miyata H, Shimada K, Fujihara Y, Tokuhiro K, Garcia T X, Matzuk M M, Ikawa M Asian J. Androl., (2021) doi: 10.4103/aja.aja_63_21. 34290169 |
Health Report | |
|---|---|
| Examination Date / Room / Rack | 2023/04/10Room:3-BRack:K 2023/01/10Room:3-BRack:K 2022/10/11Room:3-BRack:K |
Gene | |||||||
|---|---|---|---|---|---|---|---|
| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| Rasef | RAS and EF hand domain containing | 4 | Rasef | endonuclease-mediated mutation 1, Research Institute for Microbial Diseases, Osaka University | |||
Phenotype | |
|---|---|
| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
|---|---|
| Donor DNA | Streptococcus pyogenes crRNA, tracrRNA, Mouse a part of Rasef gene |
| Research application | |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Asian J Androl. Jul 16.(2021) doi: 10.4103/aja.aja_63_21.RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again. |
| Depositor | Masahito Ikawa (Osaka University) |
| Strain Status |  Frozen embryos Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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| No Data |