Strain Data Sheet

RBRC11258

Information1

Image
BRC No.RBRC11258
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameB6D2-Tmem168<em1(R539Q)Osb>
Former Common nameTmem168<R539Q/wt>
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2020. Fertilized eggs derived from BDF1 × BDF1 were used to generate this line. Mixed genetic background.
Strain descriptionTmem168 mutant (R539Q) mouse generated by the CRISPR/Cas9. 1,291 bp deletion and 11 bp deletion in the Tmem249 gene.
Colony maintenance
ReferencesFASEB J., 34(5):6399-6417 (2020). doi: 10.1096/fj.201902991R. Epub 2020 Mar 16 32175648

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Tmem168transmembrane protein 1686Tmem168<em1(R539Q)Osb>endonuclease-mediated mutation 1, Research Institute for Microbial Diseases, Osaka University

Information2

Donor DNAStreptococcus pyogenes crRNA, tracrRNA, Mouse a part of Tmem168 gene
Research application
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. FASEB J. 2020 May;34(5):6399-6417. doi: 10.1096/fj.201902991R. Epub 2020 Mar 16RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
DepositorMasahito Ikawa (Osaka University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data