Strain Data Sheet

RBRC11238

Information1

Image
BRC No.RBRC11238
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameB6D2-Cib4<em1Osb>
Former Common nameCib4<wt/-7>
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2020. Fertilized eggs derived from BDF1 × BDF1 were used to generate this line. Mixed genetic background. Homozygous males are infertile.
Strain descriptionCib4 knockout mouse generated by the CRISPR/Cas9. 7 bp deletion in the Cib4 gene.
Colony maintenance
ReferencesBiol. Reprod., Aug 4;103(2):235-243 (2020). doi: 10.1093/biolre/ioaa059 32430498

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Cib4calcium and integrin binding family member 45Cib4<em1Osb>endonuclease-mediated mutation 1, Research Institute for Microbial Diseases, Osaka University

Information2

Donor DNAhuman hU6 promoter, CMV CMV enhancer (CBh), chicken chicken beta-actin promoter (CBh), SV40 SV40 nuclear localization signal, Streptococcus pyogenes SpCas9 (human codon-optimized), crRNA, tracrRNA, Escherichia coli ampicillin resistant gene, Mouse a part of Cib4 gene
Research application
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Biol Reprod. 2020 Aug 4;103(2):235-243. doi: 10.1093/biolre/ioaa059RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
DepositorMasahito Ikawa (Osaka University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data