Strain Data Sheet

RBRC11237

Information1

Image
BRC No.RBRC11237
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameB6D2-Rsph6a<em3Osb>
Former Common nameRsph6a<em3/wt>
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2020. Fertilized eggs derived from BDF1 × BDF1 were used to generate this line. Mixed genetic background.
Strain descriptionRsph6a mutant (S17A, S20A) generated by the CRISPR/Cas9.
Colony maintenance
ReferencesBiol. Reprod. Sep 9 (2020);ioaa161. doi: 10.1093/biolre/ioaa161. Online ahead of print 32901808

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Rsph6aradial spoke head 6 homolog A (Chlamydomonas)7Rsph6a<em3Osb>endonuclease-mediated mutation 3, Research Institute for Microbial Diseases, Osaka University

Information2

Donor DNAStreptococcus pyogenes crRNA, tracrRNA, Mousea part of Rsph6a gene
Research application
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Biol Reprod. 2020 Sep 9;ioaa161. doi: 10.1093/biolre/ioaa161. Online ahead of printRECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
DepositorMasahito Ikawa (Osaka University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data