Strain Data Sheet

RBRC11232

Information1

Image
BRC No.RBRC11232
TypeCRISPR/Cas9 (Transgene)Cartagena
SpeciesMus musculus
Strain nameStock Llcfc1<em2Osb> Tg(Clgn-Llcfc1/PA)1Osb
Former Common nameLlcfc1<-1024/-1024>, Llcfc1-PA Tg, SOF1
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2020. Mixed genetic background.
Strain descriptionLlcfc1 mutant mouse generated by the CRISPR/Cas9. 1024 bp deletion in the Llcfc1 gene. This strain also has a transgene expressing Llcfc1-PA under the control of Clgn promoter.
Colony maintenance
ReferencesProc. Natl. Acad. Sci. USA, 117(21):11493-11502 (2020). doi: 10.1073/pnas.1922650117. Epub 2020 May 11 32393636

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Llcfc1LLLL and CFNLAS motif containing 16Llcfc1<em2Osb>endonuclease-mediated mutation 2, Research Institute for Microbial Diseases, Osaka University1700034O15Rik, SOF1

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
HBB2rabbit beta-globin polyA signalUNHBB2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Llcfc1LLLL and CFNLAS motif containing 1UNLlcfc1mouse Clgn promoter

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
PAPA tag (GVAMPGAEDDVV) (synthetic) human PodoplaninUNPA

Information2

Donor DNAMouse calmegin promoter, Mouse Llcfc1 cDNA, synthetic PA tag, Rabbit beta-globin polyadenylation signal
Research application
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc Natl Acad Sci U S A. 2020 May 26;117(21):11493-11502. doi: 10.1073/pnas.1922650117. Epub 2020 May 11RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
DepositorMasahito Ikawa (Osaka University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data