Strain Data Sheet

RBRC11231

Strain Information

Image
BRC No.RBRC11231
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameB6D2-Gm1673<em1Osb>
Former Common nameGm1673<-1862/wt>
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2020. Fertilized eggs derived from BDF1 × BDF1 were used to generate this line. Mixed genetic background. Homozygous males tend to be infertile.
Strain descriptionGm1673 KO mouse generated by the CRISPR/Cas9. 1862 bp deletion in the Gm1673 gene.
Colony maintenance
References
A small secreted protein NICOL regulates lumicrine-mediated sperm maturation and male fertility.
Daiji Kiyozumi, Kentaro Shimada, Michael Chalick, Chihiro Emori, Mayo Kodani, Seiya Oura, Taichi Noda, Tsutomu Endo, Martin M Matzuk, Daniel H Wreschner, Masahito Ikawa
Nat. Commun., 14(1):2354 (2023). doi: 10.1038/s41467-023-37984-x. 37095084

Health Report

Examination Date / Room / Rack2024/05/27Room:4-CRack:I
2024/02/26Room:4-CRack:I

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Gm1673predicted gene, 16735Gm1673endonuclease-mediated mutation 1, Research Institute for Microbial Diseases, Osaka University

Phenotype

Annotation by Mammalian phenotyhpe ontology
Detailed phenotype data

Ordering Information

Donor DNAStreptococcus pyogenes crRNA, tracrRNA, Mouse a part of Gm1673 gene
Research application
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nat. Commun., 14(1):2354 (2023). doi: 10.1038/s41467-023-37984-x.RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
DepositorMasahito Ikawa (Osaka University)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Live mouse (3 to 6 months)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data