Strain Data Sheet

RBRC11223

Information1

Image
BRC No.RBRC11223
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameSTOCK Del(2Cstl1-Cstdc2)1Osb
Former Common name(Cstl1-Cstdc2)<del/wt>
H-2 Haplotype
ES Cell lineEGR-G01 [129S2 x C57BL/6NCr-Tg(CAG/Acr-EGFP)]
Background strain
Appearance
Strain developmentDeposited by Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2020. EGR-G01 ES cells derived from (129S2 x B6Cr)F1 were used. Mice were crossed to BDF1. Mixed genetic background.
Strain descriptionDeletion mutant of 114 kb on chromosome 2 covering Cstl1, Cst11, Cstdc1, Cst12, Cst8, Cst13, Cst9 and Cstdc2.
Colony maintenance
ReferencesProc. Natl. Acad. Sci. USA, 116(37):18498-18506 (2019). doi: 10.1073/pnas.1908736116. Epub 2019 Aug 27 31455729

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Cstdc2cystatin domain containing 22Cstdc2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Cstl1cystatin-like 12Cstl1

Information2

Donor DNAhuman hU6 promoter, CMV CMV enhancer (CBh), chicken chicken beta-actin promoter (CBh), SV40 SV40 nuclear localization signal, Streptococcus pyogenes SpCas9 (human codon-optimized), Escherichia coli ampicillin resistant gene, Streptomyces alboniger puromycin resistant gene, Mouse a part of Cstl1 gene,Mouse a part of Cstdc2 gene
Research application
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc Natl Acad Sci U S A. 2019 Sep 10;116(37):18498-18506. doi: 10.1073/pnas.1908736116. Epub 2019 Aug 27RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
DepositorMasahito Ikawa (Osaka University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data