Strain Data Sheet

RBRC11222

Information1

Image
BRC No.RBRC11222
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6D2-Tg(Clgn-Tesmin/TurboID/3xFLAG)1Osb
Former Common nameTesmin-TurboID Tg
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2020. Fertilized eggs derived from BDF1 × BDF1 were used to generate this line. Mixed genetic background.
Strain descriptionTransgenic mice expressing Tesmin fused with TurboID and 3xFLAG under the control of mouse Clgn promoter.
Colony maintenance
ReferencesBiol. Reprod., 102(4):975-983 (2020). doi: 10.1093/biolre/ioaa002 31916570

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
FLAGFLAG tag (synthetic)UNFLAG

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
HBB2rabbit beta-globin polyA signalUNHBB2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Tesmintestis expressed metallothionein likeUNTesminMtl5mouse Clgn promoter

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
TurboIDbiotin ligase with 15 mutations relative to wild-type BirA (E. coli)UNTurboID

Information2

Donor DNAmouse Clgn promoter, mouse Tesmin cDNA, Escherichia coli Biotin ligase (BirA Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V160A, T192A, K194I, M209V, M241T, S263P, I305V), synthetic Flag tag, rabbit globin polyA signal
Research application
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Biol Reprod. 2020 Apr 15;102(4):975-983. doi: 10.1093/biolre/ioaa002RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
DepositorMasahito Ikawa (Osaka University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data