Strain Data Sheet
RBRC11222
Strain Information | |
|---|---|
| Image | |
| BRC No. | RBRC11222 |
| Type | Transgene |
| Species | Mus musculus |
| Strain name | B6D2-Tg(Clgn-Tesmin/TurboID/3xFLAG)1Osb |
| Former Common name | Tesmin-TurboID Tg |
| H-2 Haplotype | |
| ES Cell line | |
| Background strain | |
| Appearance | |
| Strain development | Deposited by Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2020. Fertilized eggs derived from BDF1 × BDF1 were used to generate this line. Mixed genetic background. |
| Strain description | Transgenic mice expressing Tesmin fused with TurboID and 3xFLAG under the control of mouse Clgn promoter. |
| Colony maintenance | |
| References | Proximity-dependent biotin labeling in testicular germ cells identified TESMIN-associated proteins. Seiya Oura, Akinori Ninomiya, Fuminori Sugihara, Martin M Matzuk, Masahito Ikawa Sci. Rep., 12(1):22198 (2022). 36564444 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |||||||
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| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| FLAG | FLAG tag (synthetic) | UN | FLAG | ||||
| HBB2 | rabbit beta-globin polyA signal | UN | HBB2 | ||||
| Tesmin | testis expressed metallothionein like | UN | Tesmin | mouse Clgn promoter | |||
| TurboID | biotin ligase with 15 mutations relative to wild-type BirA (E. coli) | UN | TurboID | ||||
Phenotype | |
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| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
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| Donor DNA | mouse Clgn promoter, mouse Tesmin cDNA, Escherichia coli Biotin ligase (BirA Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V160A, T192A, K194I, M209V, M241T, S263P, I305V), synthetic Flag tag, rabbit globin polyA signal |
| Research application | |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Sci. Rep., 12(1):22198 (2022).RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again. |
| Depositor | Masahito Ikawa (Osaka University) |
| Strain Status |  Frozen sperm |
| Strain Availability |  Recovery and QC required prior to distribution |
| Additional Info. | Necessary documents for ordering:
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BRC mice in Publications |
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| No Data |