Strain Data Sheet

RBRC11202

Strain Information

Image
BRC No.RBRC11202
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameSTOCK Slc12a2<tm1.1(tetO)Kftnk>
Former Common nameNKCC1 tetO knockin
H-2 Haplotype
ES Cell lineG0G1
Background strain
Appearance
Strain developmentDeposited by Kenji Takana, Keio University in 2021. Mixed genetic background.
Strain descriptionTetO cassette knock in at the Slc12a2 genomic area between its promoter and coding sequence.
Colony maintenance
References
Time-controllable Nkcc1 knockdown replicates reversible hearing loss in postnatal mice.
Watabe T, Xu M, Watanabe M, Nabekura J, Higuchi T, Hori K, Sato M P, Nin F, Hibino H, Ogawa K, Masuda M, Tanaka K F
Sci. Rep., 7(1):13605. (2017). 29051615

Oligodendrocytic Na+-K+-Cl- co-transporter 1 activity facilitates axonal conduction and restores plasticity in the adult mouse brain.
Yoshihiko Yamazaki, Yoshifumi Abe, Satoshi Fujii, Kenji F Tanaka
Nat. Commun., 12(1) 5146 (2021). 34446732

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frt yeast FRT (flippase recombination target) site18Frt
Slc12a2
MGI:101924
solute carrier family 12, member 218Slc12a2<tm1.1(tetO)Kftnk> targeted mutation 1.1, Kenji Tanaka
  • Delpire-McNeill syndrome(MedGEN)

  • hearing loss, autosomal dominant 78(MedGEN)

  • Kilquist syndrome(MedGEN)
  • loxP phage P1 loxP18loxP
    loxP phage P1 loxP18loxP
    tetO tetracycline operator (E. coli)18tetO

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
    Detailed phenotype data

    Ordering Information

    Donor DNAphage P1 loxP sites, yeast FRT (flippase recombination target) site, tetracycline operator (E. coli), Cytomegalovirus CMV minimum promoter, mouse Slc12a2 genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Tet system
    Specific Term and ConditionsNo specific terms and conditions. (The DEPOSITOR waives its own rights under any patents, intellectual property, or other proprietary rights with respect to the results to be obtained by use of the BIOLOGICAL RESOURCE.)
    DepositorKenji Tanaka (Keio University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data