Strain Data Sheet

RBRC11202

Information1

Image
BRC No.RBRC11202
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameSTOCK Slc12a2<tm1.1(tetO)Kftnk>
Former Common nameNKCC1 tetO knockin
H-2 Haplotype
ES Cell lineG0G1
Background strain
Appearance
Strain developmentDeposited by Kenji Takana, Keio University in 2021. Mixed genetic background.
Strain descriptionTetO cassette knock in at the Slc12a2 genomic area between its promoter and coding sequence.
Colony maintenance
ReferencesSci. Rep., 7(1):13605. (2017). 29051615

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Slc12a2solute carrier family 12, member 218Slc12a2<tm1.1(tetO)Kftnk>targeted mutation 1.1, Kenji Tanaka

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site18Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP18loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP18loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
tetOtetracycline operator (E. coli)18tetO

Information2

Donor DNAphage P1 loxP sites, yeast FRT (flippase recombination target) site, tetracycline operator (E. coli), Cytomegalovirus CMV minimum promoter, mouse Slc12a2 genomic DNA
Research applicationCre/loxP system
FLP/frt system
Tet system
Specific Term and ConditionsNo specific terms and conditions. (The DEPOSITOR waives its own rights under any patents, intellectual property, or other proprietary rights with respect to the results to be obtained by use of the BIOLOGICAL RESOURCE.)
DepositorKenji Tanaka (Keio University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data