Strain Data Sheet

RBRC11200

Information1

Image
BRC No.RBRC11200
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Bdnf<tm1(STOP-tetO)Kftnk>
Former Common nameBDNF STOP-tetO knockin
H-2 Haplotype
ES Cell lineG0G1
Background strain
Appearance
Strain developmentDeposited by Kenji Tanaka, Keio University in 2020. Mice were generated using ES cells derived from 129 x B6. Mice were further backcrossed to C57BL/6J for more than 5 generations.
Strain descriptionSTOP-tetO cassette knock in at the exon 4 of the Bdnf gene.
Colony maintenance
ReferencesSci. Rep., 9(1):3422. (2019). 30833600

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Bdnfbrain derived neurotrophic factor2Bdnf<tm1(tetO)Kftnk>targeted mutation 1, Kenji Tanaka

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site2Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP2loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)2mouse Phosphoglycerate kinase promoter (PGK promoter), E. coli EM7 promoter

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
tetOtetracycline operator (E. coli)2tetO

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyA2

Information2

Donor DNAマウス PGK プロモーター, 大腸菌 EM7プロモーター, 大腸菌 Neo 耐性遺伝子, 酵母His3 3’UTR, SV40 ウイルス SV40 polyA シグナル, 酵母 FRT配列, 大腸菌Tetracycline operator site, サイトメガロウイルス CMV最小プロモーター, P1ファージ loxP配列
Research applicationCre/loxP system
FLP/frt system
Tet system
Specific Term and ConditionsNo specific terms and conditions. (The DEPOSITOR waives its own rights under any patents, intellectual property, or other proprietary rights with respect to the results to be obtained by use of the BIOLOGICAL RESOURCE.)
DepositorKenji Tanaka (Keio University)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data