Strain Data Sheet

RBRC11198

Information1

Image
BRC No.RBRC11198
TypeCRISPR/Cas9 (Transgene)Cartagena
SpeciesMus musculus
Strain nameB6;Cg-Cdc20<em1(R286S)Jko>
Former Common nameCdc20 p.R286S knockin mouse
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Ryoji Kubo, Keio University School of Medicine in 2021. This strain was generated using fertilized eggs derived from B6D2F2 and mice were backcrossed to C57BL/6N for three generations. Homozygous mutants are lethal.
Strain descriptionCdc20 (Cell division cycle 20) mutant mice harboring a point mutation (R286S). Homozygous mutants look normal.
Colony maintenance
ReferencesAging Cell, 19(11):e13251 (2020). 33094908

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Cdc20cell division cycle 204Cdc20<em1(R286S)Jko>

Information2

Donor DNA
Research application
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Aging Cell, 19(11) e13251 (2020).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
DepositorAkiharu KUBO (Keio University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data