Strain Data Sheet
RBRC11193
Strain Information | |
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| Image | |
| BRC No. | RBRC11193 |
| Type | Targeted Mutation Congenic |
| Species | Mus musculus |
| Strain name | B6.Cg-Gt(ROSA)26Sor<tm2(Asxl1<*>)Toki> |
| Former Common name | Asxl1-MT KI |
| H-2 Haplotype | |
| ES Cell line | KY1.1[(C57BL/6J x 129S6/SvEvTac)F1] |
| Background strain | |
| Appearance | |
| Strain development | Deposited by Toshio Kitamura in 2020. KY1.1 ES cells derived from (C57BL/6J x 129S6/SvEvTac)F1 were used. Mice were further backcrossed to C57BL/6J for 6 generations. C57BL/6J congenic line. |
| Strain description | Knockin mouse strain at the ROSA26 locus in which Asxl1-MT IRES EGFP can be overexpressed in a Cre-dependent manner. |
| Colony maintenance | |
| References | Expression of mutant Asxl1 perturbs hematopoiesis and promotes susceptibility to leukemic transformation. Nagase R, Inoue D, Pastore A, Fujino T, Hou H A, Yamasaki N, Goyama S, Saika M, Kanai A, Sera Y, Horikawa S, Ota Y, Asada S, Hayashi Y, Kawabata K C, Takeda R, Tien H F, Honda H, Abdel-Wahab O, Kitamura T J. Exp. Med., 215(6):1729-1747 (2018). 29643185 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |||||||
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| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| SV40 virus Stop | 6 | ||||||
| Asxl1 | ASXL transcriptional regulator 1 | 6 | Asxl1 | ||||
| EGFP | Enhanced Green Fluorescent Protein (Aequorea victoria) | 6 | EGFP | ||||
| FLAG | FLAG tag (synthetic) | 6 | FLAG | ||||
| Frt | yeast FRT (flippase recombination target) site | 6 | Frt | ||||
| Frt | yeast FRT (flippase recombination target) site | 6 | Frt | ||||
| Gt(ROSA)26Sor | gene trap ROSA 26, Philippe Soriano | 6 | Gt(ROSA)26Sor | targeted mutation 2, Toshio Kitamura | |||
| Ires | internal ribosomal entry site (EMCV) | 6 | |||||
| SA | Splice acceptor (adenovirus) | 6 | SA | ||||
| loxP | phage P1 loxP | 6 | loxP | ||||
| loxP | phage P1 loxP | 6 | loxP | ||||
| neo | neomycin resistance gene (E. coli) | 6 | herpes simplex virus thymidine kinase promoter (HSV tk promoter) | ||||
Phenotype | |
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| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
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| Donor DNA | SA (Adenovirus Splicing acceptor), P1 phage loxP sites, herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli Neomycin resistance gene, SV40 virus Stop, FLAG tag (synthetic), Mouse Asxl1 (1-1,890) cDNA, yeast FRT (flippase recombination target) sites, IRES (internal ribosomal entry site (EMCV)), jellyfish EGFP cDNA, bovine growth hormone polyA, mouse Gt(ROSA)26Sor genomic DNA |
| Research application | Cre/loxP system FLP/frt system Fluorescent Proteins/lacZ System |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J Exp Med. Jun 4;215(6):1729-1747 (2018)For use of the BIOLOGICAL RESOURCE by a company and a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. |
| Depositor | Toshio Kitamura (The University of Tokyo) |
| Strain Status |  Frozen embryos Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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| No Data |