Strain Data Sheet

RBRC11193

Information1

Image
BRC No.RBRC11193
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Gt(ROSA)26Sor<tm2(Asxl1<*>)Toki>
Former Common nameAsxl1-MT KI
H-2 Haplotype
ES Cell lineKY1.1[(C57BL/6J x 129S6/SvEvTac)F1]
Background strain
Appearance
Strain developmentDeposited by Toshio Kitamura in 2020. KY1.1 ES cells derived from (C57BL/6J x 129S6/SvEvTac)F1 were used. Mice were further backcrossed to C57BL/6J for 6 generations. C57BL/6J congenic line.
Strain descriptionKnockin mouse strain at the ROSA26 locus in which Asxl1-MT IRES EGFP can be overexpressed in a Cre-dependent manner.
Colony maintenance
ReferencesJ. Exp. Med., 215(6):1729-1747 (2018). 29643185

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Gt(ROSA)26Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm2(Asxl1<*>)Toki>targeted mutation 2, Toshio Kitamura

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Asxl1additional sex combs like 16Asxl1

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)6EGFP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
FLAGFLAG tag (synthetic)6FLAG

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site6Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site6Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Iresinternal ribosomal entry site (EMCV)6

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SAadenovirus splice acceptor sequence (SA)6SA

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)6herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 virus Stop6

Information2

Donor DNASA (Adenovirus Splicing acceptor), P1 phage loxP sites, herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli Neomycin resistance gene, SV40 virus Stop, FLAG tag (synthetic), Mouse Asxl1 (1-1,890) cDNA, yeast FRT (flippase recombination target) sites, IRES (internal ribosomal entry site (EMCV)), jellyfish EGFP cDNA, bovine growth hormone polyA, mouse Gt(ROSA)26Sor genomic DNA
Research applicationCre/loxP system
FLP/frt system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J Exp Med. Jun 4;215(6):1729-1747 (2018)For use of the BIOLOGICAL RESOURCE by a company and a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
DepositorToshio Kitamura (The University of Tokyo)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)
  4. GFP Transfer License (Japanese / English)
    Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.

Genotyping protocol -PCR-

BRC mice in Publications

No Data