Strain Data Sheet

RBRC11185

Strain Information

Image
BRC No.RBRC11185
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Gt(ROSA)26Sor<tm1.1(CAG-tdiRFP713NLS)Imayo>
Former Common nameR26-CAG-LoxP-iRFP713NLS
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Itaru Imayoshi, Kyoto University in 2021 and generated in collaboration with Hiroyuki Okuno, Kagoshima University. EGR-101 ES cells derived from C57BL/6N were used to generate this strain.
Strain descriptiontdiFP713NLS fluorescent protein is expressed after both Cre-and FLP-recombination. The transgene construct is knocked into Rosa26-locus. To achieve high-level transgene expression, tdiFP713NLS is expressed under CAG promoter and WPRE sequence is inserted into 3’ UTR region. FRT-Stop-FRT cassette was removed by crossing pCAG-FLPe strain. The HA-epitope tga was is attched to tdiFP713NLS.
Colony maintenance
References

Health Report

Examination Date / Room / Rack2024/06/10Room:3-2Rack:D
2024/03/11Room:3-2Rack:D
2023/12/11Room:3-2Rack:D
2023/09/11Room:3-2Rack:D
2023/06/13Room:3-2Rack:D
2023/03/13Room:3-2Rack:D
2022/12/12Room:3-2Rack:D

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Gt(ROSA)26Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm1.1(CAG-tdiRFP713NLS)Imayo>targeted mutation 1.1, Itaru Imayoshi

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site6Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GHGrowth hormone polyA (Bovine)6GH

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
HAhuman influenza virus HA (hemagglutinin) tag sequence6HA

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
T2AThosea asigna virus 2A peptide (insect)6T2A

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
WPREWoodchuck hepatitis virus posy-transcriptional regulatory element (WPRE)6WPRE

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
iRFP713iRFP713 (humanized iRFP713)6iRFP713

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
iRFP713iRFP713 (humanized iRFP713)6iRFP713

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)6mouse Phosphoglycerate kinase promoter (PGK promoter), phage T7 gb2 promoter

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
nlsSV40 nuclear localaization signal (chemically-synthesized nucleotide)6nls

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
nlsSV40 nuclear localaization signal (chemically-synthesized nucleotide)6nls

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
tdiRFP713tdiRFP713 (tandem dimeric iRFP713)6tdiRFP713CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyA6

Ordering Information

Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, chicken beta-actin intron, rabbit beta-globin intron), Bacteriophage P1 loxP sites, mouse Phosphoglycerate kinase promoter (PGK promoter), phage T7 gb2 promoter, E. coli neomycin resistance gene, pA, bovine GH polyA, SV40 polyA signal, rabbit beta-globin polyA, Yeast FRT (flippase recombination target) site, Near-InfraRed Fluorescent Protein (Rhodopseudomonas palustris) tdiRFP713 (tandem dimeric iRFP713), SV40 nuclear localaization signal (chemically-synthesized nucleotide), Thosea asigna virus 2A peptide (insect), human influenza virus HA (hemagglutinin) tag sequence, Woodchuck hepatitis virus posy-transcriptional regulatory element (WPRE)
Research applicationCre/loxP system
FLP/frt system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (will be announced.) The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from the DEPOSITOR Dr. Itaru Imayoshi (E-mail:imayoshi.itaru.2n@kyoto-u.ac.jp). (No approval form (Form D) is necessary).
DepositorItaru Imayoshi (Kyoto University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. CAGGS MTA (English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data