Strain Data Sheet

RBRC11179

Information1

Image
BRC No.RBRC11179
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Gt(ROSA)26Sor<tm1.1(CAG-tdTomato)Imayo>
Former Common nameR26-CAG-LoxP-tdTom
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Itaru Imayoshi, Kyoto University in 2021 and generated in collaboration with Hiroyuki Okuno, Kagoshima University. EGR-101 ES cells derived from C57BL/6N were used to generate this strain.
Strain descriptiontdTomato fluorescent protein is expressed after both Cre-and FLP-recombination. The transgene construct is knocked into Rosa26-locus. To achieve high-level transgene expression, tdTomato is expressed under CAG promoter and WPRE sequence is inserted into 3’ UTR region. FRT-Stop-FRT cassette was removed by crossing pCAG-FLPe strain.
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Gt(ROSA)26Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm1.1(CAG-tdTomato)Imayo>targeted mutation 1.1, Itaru Imayoshi

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site6Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GHGrowth hormone polyA (Bovine)6GH

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
T2AThosea asigna virus 2A peptide (insect)6T2A

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
WPREWoodchuck hepatitis virus posy-transcriptional regulatory element (WPRE)6WPRE

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)6mouse Phosphoglycerate kinase promoter (PGK promoter), gb2 promoter

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
tdTomatotandem dimeric Red Fluorescent Protein (Discosoma sp.)6tdTomatoCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Information2

Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, chicken beta-actin intron, rabbit beta-globin intron), Bacteriophage P1 loxP sites, mouse Phosphoglycerate kinase promoter (PGK promoter), gb2 promoter, E.coli neo, Bovine Growth hormone polyA, Yeast FRT (flippase recombination target) site, Woodchuck hepatitis virus posy-transcriptional regulatory element (WPRE), Red Fluorescent Protein (Discosoma sp.) tdTomato (tandem dimeric Tomato), T2A (Thosea asigna virus 2A peptide (insect))
Research applicationCre/loxP system
FLP/frt system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. 後日指定The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from the DEPOSITOR Dr. Itaru Imayoshi (E-mail:imayoshi.itaru.2n@kyoto-u.ac.jp). (No approval form (Form D) is necessary).
DepositorItaru Imayoshi (Kyoto University)
Strain Statusan icon for Live miceLive mice
Strain AvailabilityLive mouse (3 to 6 months)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: FP license of TBUSA
  3. CAGGS MTA (English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data