Strain Data Sheet

RBRC11175

Strain Information

Image
BRC No.RBRC11175
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Prdm16<tm1Kmori>
Former Common nameMEL1, PRDM16
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeposited by Kazuhiro Morishitam, Miyazaki University in 2020. Mice were generated using TT2 ES cells derived from (C57BL/6 x CBA)F1. Mice were further backcrossed to C57BL/6N for more than 10 generations. C57BL/6 genetic background.
Strain descriptionPrdm16 knockout mice. A neo gene fragment replaced exon 6 of the Prdm16 gene.
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Prdm16PR domain containing 164Prdm16targeted mutation 1, Kazuhiro Morishita
  • left ventricular noncompaction 8(MedGEN)
    • neoneomycin resistance gene (E. coli)4mouse phosphoglycerate kinase promoter (PGK promoter)
  • left ventricular noncompaction 8(MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
    Detailed phenotype data

    Ordering Information

    Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), E.coli neomycin resistance gene, mouse Prdm16 genomic DNA
    Research application
    Specific Term and ConditionsFor use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
    DepositorKazuhiro Morishita (University of Miyazaki)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data