Strain Data Sheet

RBRC11164

Strain Information

Image
BRC No.RBRC11164
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC.Cg-Spib<tm1Ksho>
Former Common nameSpi-B KO BALB
H-2 Haplotype
ES Cell lineBruce 4 [B6.Cg-Thy1<a>]
Background strain
Appearance
Strain developmentDeveloped by Tsuneyasu Kaisho, Research Center for Allergy and Immunologuy, RIKEN around 2005.
Strain descriptionSpi-B is an Ets family transcription factor. In C.Cg-Spib<tm1Ksho> mice, the Spi-B gene is targeted and disrupted. Spi-B-deficient mice show defects in plasmacytoid dendritic cell function and development and in development of an intestinal epithelial cell subset, M cell.
Colony maintenance
References
Spi-B is critical for plasmacytoid dendritic cell function and development.
Sasaki I, Hoshino K, Sugiyama T, Yamazaki C, Yano T, Iizuka A, Hemmi H, Tanaka T, Saito M, Sugiyama M, Fukuda Y, Ohta T, Sato K, Ainai A, Suzuki T, Hasegawa H, Toyama-Sorimachi N, Kohara H, Nagasawa T, Kaisho T
Blood, 120(24):4733-43 (2012). 23065153

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
SpibSpi-B transcription factor (Spi-1/PU.1 related)7Spibtargeted mutation 1, Tsuneyasu Kaisho
neoneomycin resistance gene (E. coli)7herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
Detailed phenotype data

Ordering Information

Donor DNAPolyoma virus Enhancer (MC1), HSV TK promoter (MC1), Escherichia coli Neomycin resistance gene, HSV TK polyA signal, mouse Spib genomic DNA
Research applicationImmunology and Inflammation Research
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Blood. 2012 Dec 6;120(24):4733-43.
DepositorTsuneyasu Kaisho (RIKEN)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data