Strain Data Sheet
RBRC11163
Strain Information | |
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| Image | |
| BRC No. | RBRC11163 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | B6.Cg-Spib<tm1Ksho> |
| Former Common name | Spi-B KO B6 |
| H-2 Haplotype | |
| ES Cell line | Bruce 4 [B6.Cg-Thy1<a>] |
| Background strain | |
| Appearance | |
| Strain development | Developed by Tsuneyasu Kaisho, Research Center for Allergy and Immunologuy, RIKEN around 2005. |
| Strain description | Spi-B is an Ets family transcription factor. In B6.Cg-Spib<tm1Ksho> mice, the Spi-B gene is targeted and disrupted. Spi-B-deficient mice show defects in plasmacytoid dendritic cell function and development and in development of an intestinal epithelial cell subset, M cell. |
| Colony maintenance | |
| References | Spi-B is critical for plasmacytoid dendritic cell function and development. Sasaki I, Hoshino K, Sugiyama T, Yamazaki C, Yano T, Iizuka A, Hemmi H, Tanaka T, Saito M, Sugiyama M, Fukuda Y, Ohta T, Sato K, Ainai A, Suzuki T, Hasegawa H, Toyama-Sorimachi N, Kohara H, Nagasawa T, Kaisho T Blood, 120(24):4733-43 (2012). 23065153 |
Health Report | |
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| Examination Date / Room / Rack | 2024/05/27Room:4-CRack:L 2024/02/26Room:4-CRack:L 2023/11/27Room:4-CRack:L 2023/08/28Room:4-CRack:L 2023/05/29Room:4-CRack:L 2023/05/22Room:4-CRack:L |
Gene | |||||||
|---|---|---|---|---|---|---|---|
| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| Spib | Spi-B transcription factor (Spi-1/PU.1 related) | 7 | Spib | targeted mutation 1, Tsuneyasu Kaisho | |||
| neo | neomycin resistance gene (E. coli) | 7 | herpes simplex virus thymidine kinase promoter (HSV tk promoter) | ||||
Phenotype | |
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| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
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| Donor DNA | Polyoma virus Enhancer (MC1), HSV TK promoter (MC1), Escherichia coli Neomycin resistance gene, HSV TK polyA signal, mouse Spib genomic DNA |
| Research application | Immunology and Inflammation Research |
| Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Blood, 120(24):4733-43 (2012).The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. |
| Depositor | Tsuneyasu Kaisho (RIKEN) |
| Strain Status |  Frozen embryos Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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| No Data |