Strain Data Sheet



BRC No.RBRC11163
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Spib<tm1Ksho>
Former Common nameSpi-B KO B6
H-2 Haplotype
ES Cell lineBruce 4 [B6.Cg-Thy1<a>]
Background strain
Strain developmentDeveloped by Tsuneyasu Kaisho, Research Center for Allergy and Immunologuy, RIKEN around 2005.
Strain descriptionSpi-B is an Ets family transcription factor. In B6.Cg-Spib<tm1Ksho> mice, the Spi-B gene is targeted and disrupted. Spi-B-deficient mice show defects in plasmacytoid dendritic cell function and development and in development of an intestinal epithelial cell subset, M cell.
Colony maintenance
ReferencesBlood, 120(24):4733-43 (2012). 23065153

Health Report

Examination Date / Room / Rack


Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SpibSpi-B transcription factor (Spi-1/PU.1 related)7Spib<tm1Ksho>targeted mutation 1, Tsuneyasu Kaisho

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)7herpes simplex virus thymidine kinase promoter (HSV tk promoter)


Donor DNAPolyoma virus Enhancer (MC1), HSV TK promoter (MC1), Escherichia coli Neomycin resistance gene, HSV TK polyA signal, mouse Spib genomic DNA
Research applicationImmunology and Inflammation Research
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Blood. 2012 Dec 6;120(24):4733-43.
DepositorTsuneyasu Kaisho (RIKEN)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data