Strain Data Sheet

RBRC11161

Strain Information

Image
BRC No.RBRC11161
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6N-Arg1<tm1.1(KikGR)Ksho>
Former Common nameArg1-KikGR
H-2 Haplotype
ES Cell lineJM8A3.N1 [C57BL/6N-A<tm1Brd>]
Background strain
Appearance
Strain developmentDeveloped by Tsuneyasu Kaisho, Research Center for Allergy and Immunologuy, RIKEN around 2009.
Strain descriptionArginase 1, ARG1, is an enzyme in urea cycle and generates ornithine and urea from arginine. In type 2 inflammation, ARGI expression is known to be increased in macrophages. ArgI-KikGR mice were generated by knocking a gene encoding a photoconvertible green-to-red fluorescent protein KikGR into the murine ArgI gene locus.
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Arg1arginase, liver10Arg1targeted mutation 1.1, Tsuneyasu Kaisho
  • hyperargininemia(DisGeNET, MedGEN)
    • Frtyeast FRT (flippase recombination target) site10Frt
  • hyperargininemia(DisGeNET, MedGEN)
    • GHGrowth hormone polyA (Bovine)10GH
  • hyperargininemia(DisGeNET, MedGEN)
    • kikGRGreen-Red Fluorescent Protein (Favia favus)10kikGR
  • hyperargininemia(DisGeNET, MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
    Detailed phenotype data

    Ordering Information

    Donor DNAFavia favus KikGR cDNA, Bos taurus bGH polyA, Saccharomyces cerevisiae FRT site, mouse Arg1 genomic DNA
    Research applicationFLP/frt system
    Fluorescent Proteins/lacZ System
    Immunology and Inflammation Research
    Metabolism Research
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (will be announced.) The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit organization for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and a right holder for phKikGRl-S1, Medical & Biological Laboratories (MBL) Co., Ltd., and must obtain a prior written consent from the DEPOSITOR and MBL. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEVELOPER of the kikGR using Approval form (form V) (Lab Contact: Laboratory for Cell Function Dynamics, RIKEN CBS: mta-cfds@ml.riken.jp).
    DepositorTsuneyasu Kaisho (RIKEN)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Approval form (form V) from the developer (English)
    5. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data