Strain Data Sheet

RBRC11140

Strain Information

Image
BRC No.RBRC11140
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Dnase1l3<tm1Dkit> Dffb<tm1Osa>
Former Common nameCAD-/-DNase1L3-/- double knockout mouse
H-2 Haplotype
ES Cell lineBruce 4 [B6.Cg-Thy1<a>]
Background strain
Appearance
Strain developmentDeposited by Daisuke Kitamura, Tokyo University of Science in 2020.
Strain descriptionDouble mutant of the Dnase1l3 and Dffb.
Colony maintenance
References
Cell-free DNA in blood circulation is generated by DNase1L3 and caspase-activated DNase.
Watanabe T, Takada S, Mizuta R
Biochem. Biophys. Res. Commun., 516, 790-795 (2019). 31255286

Impaired thymic development in mouse embryos deficient in apoptotic DNA degradation.
Kawane K, Fukuyama H, Yoshida H, Nagase H, Ohsawa Y, Uchiyama Y, Okada K, Iida T, Nagata S
Nat. Immunol., 4, 138-144 (2003). 12524536

DNase gamma is the effector endonuclease for internucleosomal DNA fragmentation in necrosis.
Mizuta R, Araki S, Furukawa M, Furukawa Y, Ebara S, Shiokawa D, Hayashi K, Tanuma S, Kitamura D
PLoS One, 8(12):e80223 (2013). 24312463

Health Report

Examination Date / Room / Rack2024/06/10Room:3-2Rack:A
2024/03/11Room:3-2Rack:A
2023/12/11Room:3-2Rack:A
2023/09/11Room:3-2Rack:A
2023/06/26Room:3-2Rack:A
2023/06/13Room:3-2Rack:A
2023/03/13Room:3-2Rack:A
2022/12/12Room:3-2Rack:A
2022/09/12Room:3-2Rack:A

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Dnase1l3deoxyribonuclease 1-like 314Dnase1l3targeted mutation 1, Daisuke Kitamura
  • autosomal systemic lupus erythematosus type 16(MedGEN)
    • loxPphage P1 loxP14loxP
  • autosomal systemic lupus erythematosus type 16(MedGEN)
    • loxPphage P1 loxP14loxP
  • autosomal systemic lupus erythematosus type 16(MedGEN)
    • neoneomycin resistance gene (E. coli)14mouse phosphoglycerate kinase promoter (PGK promoter)
  • autosomal systemic lupus erythematosus type 16(MedGEN)
    • DffbDNA fragmentation factor, beta subunit4Dffbtargeted mutation 1, Shigekazu Nagata
  • autosomal systemic lupus erythematosus type 16(MedGEN)
    • neoneomycin resistance gene (E. coli)4herpes simplex virus thymidine kinase promoter (HSV tk promoter)
  • autosomal systemic lupus erythematosus type 16(MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
    Detailed phenotype data

    Ordering Information

    Donor DNAP1 phage loxP sites, mouse phosphoglycerate kinase promoter (PGK promoter), E.coli Neomycin resistant gene, Mouse Pgk1 PolyA signal, Mouse DNase1l3 genomic DNA, Herpes simplex virus Thymidine kinase gene promoter, Mouse Dffb genomic DNA
    Research applicationCre/loxP system
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Biochem. Biophys. Res. Commun., 516, 790-795 (2019). Nat. Immunol., 4, 138-144 (2003).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. RECIPIENT may only use the BIOLOGICAL RESOURCE for non-commercial academic research purpose. The RECIPIENT should negotiate with the DEPOSITOR in the case of application for any patents or commercial use with the results from the use of the BIOLOGICAL RESOURCE.
    DepositorDaisuke Kitamura (Tokyo University of Science)
    Strain Statusan icon for Live miceLive mice
    an icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityCryopreserved sperm (within 1 month)
    Live mouse (1 to 3 months)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data