Strain Data Sheet

RBRC11140

Information1

Image
BRC No.RBRC11140
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Dnase1l3<tm1Dkit> Dffb<tm1Osa>
Former Common nameCAD-/-DNase1L3-/- double knockout mouse
H-2 Haplotype
ES Cell lineBruce 4 [B6.Cg-Thy1<a>]
Background strain
Appearance
Strain developmentDeposited by Daisuke Kitamura, Tokyo University of Science in 2020.
Strain descriptionDouble mutant of the Dnase1l3 and Dffb.
Colony maintenance
ReferencesNat. Immunol., 4, 138-144 (2003). 12524536
PLoS One, 8(12):e80223 (2013). 24312463
Biochem. Biophys. Res. Commun., 516, 790-795 (2019). 31255286

Health Report

Examination Date / Room / Rack2022/06/13Room:3-2Rack:A
2022/03/14Room:3-2Rack:A
2021/12/14Room:3-2Rack:A

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Dnase1l3deoxyribonuclease 1-like 314Dnase1l3<tm1Dkit>targeted mutation 1, Daisuke Kitamura

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP14loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP14loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)14mouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
DffbDNA fragmentation factor, beta subunit4Dffb<tm1Osa>targeted mutation 1, Shigekazu Nagata40kDa, 5730477D02Rik, caspase-activated DNase, CPAN, DFF40, Didff

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)4neo; neomycin;herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Information2

Donor DNAP1 phage loxP sites, mouse phosphoglycerate kinase promoter (PGK promoter), E.coli Neomycin resistant gene, Mouse Pgk1 PolyA signal, Mouse DNase1l3 genomic DNA, Herpes simplex virus Thymidine kinase gene promoter, Mouse Dffb genomic DNA
Research applicationCre/loxP system
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Biochem. Biophys. Res. Commun., 516, 790-795 (2019). Nat. Immunol., 4, 138-144 (2003).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. RECIPIENT may only use the BIOLOGICAL RESOURCE for non-commercial academic research purpose. The RECIPIENT should negotiate with the DEPOSITOR in the case of application for any patents or commercial use with the results from the use of the BIOLOGICAL RESOURCE.
DepositorDaisuke Kitamura (Tokyo University of Science)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Live mouse (1 to 3 months)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data