Strain Data Sheet

RBRC11139

Strain Information

Image
BRC No.RBRC11139
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Dnase1l3<tm1Dkit>
Former Common nameDNase1L3 (DNaseγ) knockout mouse
H-2 Haplotype
ES Cell lineBruce 4 [B6.Cg-Thy1<a>]
Background strain
Appearance
Strain developmentDeposited by Daisuke Kitamura, Tokyo University of Science in 2020. Mice were generated using Bruce 4 ES cells derived from B6.Cg-Thy1.1. C57BL/6 congenic line.
Strain descriptionDnase1l3 mutant mice. A neo fragment was inserted at the exon 5 of the Dnase1l3 gene.
Colony maintenance
References
DNase gamma is the effector endonuclease for internucleosomal DNA fragmentation in necrosis.
Mizuta R, Araki S, Furukawa M, Furukawa Y, Ebara S, Shiokawa D, Hayashi K, Tanuma S, Kitamura D
PLoS One, 8(12):e80223 (2013). 24312463

Cell-free DNA in blood circulation is generated by DNase1L3 and caspase-activated DNase.
Watanabe T, Takada S, Mizuta R
Biochem. Biophys. Res. Commun., 27;516(3):790-795 (2019). 31255286

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Dnase1l3deoxyribonuclease 1-like 314Dnase1l3targeted mutation 1, Daisuke Kitamura
  • autosomal systemic lupus erythematosus type 16(MedGEN)
    • loxPphage P1 loxP14loxP
  • autosomal systemic lupus erythematosus type 16(MedGEN)
    • loxPphage P1 loxP14loxP
  • autosomal systemic lupus erythematosus type 16(MedGEN)
    • neoneomycin resistance gene (E. coli)14mouse phosphoglycerate kinase promoter (PGK promoter)
  • autosomal systemic lupus erythematosus type 16(MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
    Detailed phenotype data

    Ordering Information

    Donor DNAP1 phage loxP sites, mouse phosphoglycerate kinase promoter (PGK promoter), E.coli Neomycin resistant gene, Mouse Pgk1 PolyA signal, Mouse DNase1l3 genomic DNA
    Research applicationCre/loxP system
    Immunology and Inflammation Research
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. PLoS One, 8(12):e80223 (2013).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
    DepositorDaisuke Kitamura (Tokyo University of Science)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data