Strain Data Sheet

RBRC11139

Information1

Image
BRC No.RBRC11139
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Dnase1l3<tm1Dkit>
Former Common nameDNase1L3 (DNaseγ) knockout mouse
H-2 Haplotype
ES Cell lineBruce 4 [B6.Cg-Thy1<a>]
Background strain
Appearance
Strain developmentDeposited by Daisuke Kitamura, Tokyo University of Science in 2020. Mice were generated using Bruce 4 ES cells derived from B6.Cg-Thy1.1. C57BL/6 congenic line.
Strain descriptionDnase1l3 mutant mice. A neo fragment was inserted at the exon 5 of the Dnase1l3 gene.
Colony maintenance
ReferencesBiochem. Biophys. Res. Commun., 27;516(3):790-795 (2019). 31255286
PLoS One, 8(12):e80223 (2013). 24312463

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Dnase1l3deoxyribonuclease 1-like 314Dnase1l3<tm1Dkit>targeted mutation 1, Daisuke Kitamura

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP14loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP14loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)14mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAP1 phage loxP sites, mouse phosphoglycerate kinase promoter (PGK promoter), E.coli Neomycin resistant gene, Mouse Pgk1 PolyA signal, Mouse DNase1l3 genomic DNA
Research applicationCre/loxP system
Immunology and Inflammation Research
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. PLoS One, 8(12):e80223 (2013).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
DepositorDaisuke Kitamura (Tokyo University of Science)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data