Strain Data Sheet

RBRC11138

Strain Information

Image
BRC No.RBRC11138
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Blnk<tm1Dkit> Ptprc<a>
Former Common nameBLNK(BASH or SLP65) knockout mouse
H-2 Haplotype
ES Cell lineBruce 4 [B6.Cg-Thy1<a>]
Background strain
Appearance
Strain developmentDeposited by Daisuke Kitamura, Tokyo University of Science in 2020. Mice were generated using Bruce 4 ES cells derived from B6.Cg-Thy1.1. C57BL/6 congenic line.
Strain descriptionC57BL/6 congenic Blnk mutant mice with Ly5.1.
Colony maintenance
References
Distinct signaling requirements for Dmu selection, IgH allelic exclusion, pre-B cell transition, and tumor suppression in B cell progenitors.
Hayashi K, Yamamoto M, Nojima T, Goitsuka R, Kitamura D
Immunity, 18(6):825-36 (2003). 12818163

The B cell-restricted adaptor BASH is required for normal development and antigen receptor-mediated activation of B cells.
Hayashi K, Nittono R, Okamoto N, Tsuji S, Hara Y, Goitsuka R, Kitamura D
Proc. Natl. Acad. Sci. USA, 97(6):2755-60 (2020). 10688901

Health Report

Examination Date / Room / Rack2024/05/07Room:2-検Rack:G
2024/02/05Room:2-検Rack:G
2023/11/06Room:2-検Rack:G
2023/08/07Room:2-検Rack:G
2023/05/08Room:2-検Rack:G
2023/02/06Room:2-検Rack:G
2022/11/07Room:2-検Rack:G

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Ptprcprotein tyrosine phosphatase receptor type C1Ptprca variant
  • agammaglobulinemia 4, autosomal recessive(MedGEN)
    • BlnkB cell linker19Blnktargeted mutation 1, Daisuke Kitamura
  • agammaglobulinemia 4, autosomal recessive(MedGEN)
    • loxPphage P1 loxP19loxP
  • agammaglobulinemia 4, autosomal recessive(MedGEN)
    • loxPphage P1 loxP19loxP
  • agammaglobulinemia 4, autosomal recessive(MedGEN)
    • neoneomycin resistance gene (E. coli)19mouse phosphoglycerate kinase promoter (PGK promoter)
  • agammaglobulinemia 4, autosomal recessive(MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
    Detailed phenotype data

    Ordering Information

    Donor DNASV40 PolyA signal, P1 phage loxP sites, mouse PGK gene promoter, E.coli Neomycin resistant gene, mouse Pgk1 PolyA signal, Mouse Blnk genomic DNA
    Research applicationCre/loxP system
    Immunology and Inflammation Research
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Immunity, 18(6):825-36 (2003).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
    DepositorDaisuke Kitamura (Tokyo University of Science)
    Strain Statusan icon for Live miceLive mice
    an icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityCryopreserved embryos (within 1 month)
    Live mouse (3 to 6 months)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data