Strain Data Sheet

RBRC11138

Information1

Image
BRC No.RBRC11138
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Blnk<tm1Dkit> Ptprc<a>
Former Common nameBLNK(BASH or SLP65) knockout mouse
H-2 Haplotype
ES Cell lineBruce 4 [B6.Cg-Thy1<a>]
Background strain
Appearance
Strain developmentDeposited by Daisuke Kitamura, Tokyo University of Science in 2020. Mice were generated using Bruce 4 ES cells derived from B6.Cg-Thy1.1. C57BL/6 congenic line.
Strain descriptionC57BL/6 congenic Blnk mutant mice with Ly5.1.
Colony maintenance
ReferencesProc. Natl. Acad. Sci. USA, 97(6):2755-60 (2020). 10688901
Immunity, 18(6):825-36 (2003). 12818163

Health Report

Examination Date / Room / Rack2022/08/08Room:2-検Rack:G
2022/05/09Room:2-検Rack:G
2022/02/21Room:2-検Rack:G

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Ptprcprotein tyrosine phosphatase, receptor type, C1Ptprc<a>a variantB220, CD45, Cd45, CD45R, Ly-5, LY-5, Lyt-4, T200

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
BlnkB-cell linker19Blnk<tm1Dkit>targeted mutation 1, Daisuke KitamuraBASH, Bca, BCA, BLNK, Ly57, Ly-57, SLP-65

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP19loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP19loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)19mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNASV40 PolyA signal, P1 phage loxP sites, mouse PGK gene promoter, E.coli Neomycin resistant gene, mouse Pgk1 PolyA signal, Mouse Blnk genomic DNA
Research applicationCre/loxP system
Immunology and Inflammation Research
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Immunity, 18(6):825-36 (2003).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
DepositorDaisuke Kitamura (Tokyo University of Science)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved embryos (within 1 month)
Live mouse (3 to 6 months)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data