Strain Data Sheet

RBRC11137

Strain Information

Image
BRC No.RBRC11137
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6J.B6N-Qrfp<tm2.1(icre)Stak>
Former Common nameQrfp-iCre
H-2 Haplotype
ES Cell lineRENKA [C57BL/6NCrlCrlj]
Background strain
Appearance
Strain developmentDeposited by Takeshi Sakurai, University of Tsukuba, 2020. Mice were generated using RENKA ES cells derived from C57BL/6NCrlCrlj. Mice were further backcrossed to C57BL/6J for 10 generations. Pgk-neo fragment was deleted from this strain.
Strain descriptioniCre knock in mice at the translation initiation codon of the Qrfp gene.
Colony maintenance
References
A discrete neuronal circuit induces a hibernation-like state in rodents.
Takahashi T M, Sunagawa G A, Soya S, Abe M, Sakurai K, Ishikawa K, Yanagisawa M, Hama H, Hasegawa E, Miyawaki A, Sakimura K, Takahashi M, Sakurai T
Nature, 583(7814):109-114 (2020). 32528181

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frt yeast FRT (flippase recombination target) site2Frt
Qrfp
MGI:3630329		pyroglutamylated RFamide peptide2Qrfp<tm2.1(icre)Stak> targeted mutation 2.1, Takeshi Sakurai
icre phage P1 codon improved Cre (iCre)2icre

Phenotype

Phenotype annotation from literatures by Mammalian phenotype ontology
Detailed phenotype data

Ordering Information

Donor DNABactriophage P1 Cre recombinase, SV40 poly A, Saccharomyces cerevisiae FRT site, mouse Qrfp genomic DNA
Research applicationCre/loxP system
FLP/frt system
Cre driver
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nature, 583(7814):109-114 (2020).
DepositorTakeshi Sakurai (University of Tsukuba)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-
Mouse of the Month Sep 2021

BRC mice in Publications

No Data