Strain Data Sheet
RBRC11137
Strain Information | |
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| Image | |
| BRC No. | RBRC11137 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | B6J.B6N-Qrfp<tm2.1(icre)Stak> |
| Former Common name | Qrfp-iCre |
| H-2 Haplotype | |
| ES Cell line | RENKA [C57BL/6NCrlCrlj] |
| Background strain | |
| Appearance | |
| Strain development | Deposited by Takeshi Sakurai, University of Tsukuba, 2020. Mice were generated using RENKA ES cells derived from C57BL/6NCrlCrlj. Mice were further backcrossed to C57BL/6J for 10 generations. Pgk-neo fragment was deleted from this strain. |
| Strain description | iCre knock in mice at the translation initiation codon of the Qrfp gene. |
| Colony maintenance | |
| References | A discrete neuronal circuit induces a hibernation-like state in rodents. Takahashi T M, Sunagawa G A, Soya S, Abe M, Sakurai K, Ishikawa K, Yanagisawa M, Hama H, Hasegawa E, Miyawaki A, Sakimura K, Takahashi M, Sakurai T Nature. 2020 Jul;583(7814):109-114. doi: 10.1038/s41586-020-2163-6. 32528181 |
Health Report | |
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| Examination Date / Room / Rack | 2023/01/23Room:3-CRack:G 2022/10/25Room:3-CRack:G |
Gene | |||||||
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| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| Frt | yeast FRT (flippase recombination target) site | 2 | Frt | ||||
| Qrfp | pyroglutamylated RFamide peptide | 2 | Qrfp | targeted mutation 2.1, Takeshi Sakurai | |||
| icre | phage P1 codon improved Cre (iCre) | 2 | icre | ||||
Phenotype | |
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| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
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| Donor DNA | Bactriophage P1 Cre recombinase, SV40 poly A, Saccharomyces cerevisiae FRT site, mouse Qrfp genomic DNA |
| Research application | Cre/loxP system FLP/frt system |
| Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nature. 2020 Jul;583(7814):109-114. doi: 10.1038/s41586-020-2163-6. |
| Depositor | Takeshi Sakurai (University of Tsukuba) |
| Strain Status |  Frozen embryos Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- Mouse of the Month Sep 2021 |
BRC mice in Publications |
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| No Data |