Strain Data Sheet

RBRC11137

Information1

Image
BRC No.RBRC11137
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6J.B6N-Qrfp<tm2.1(icre)Stak>
Former Common nameQrfp-iCre
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Takeshi Sakurai, University of Tsukuba, 2020. Mice were generated using RENKA ES cells derived from C57BL/6NCrlCrlj. Mice were further backcrossed to C57BL/6J for 10 generations. Pgk-neo fragment was deleted from this strain.
Strain descriptioniCre knock in mice at the translation initiation codon of the Qrfp gene.
Colony maintenance
ReferencesNature. 2020 Jul;583(7814):109-114. doi: 10.1038/s41586-020-2163-6. 32528181

Health Report

Examination Date / Room / Rack2022/07/25Room:3-CRack:G
2022/04/25Room:3-CRack:G
2022/02/14Room:3-CRack:G
2021/11/15Room:3-CRack:G

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Qrfppyroglutamylated RFamide peptide2Qrfp<tm2.1(icre)Stak>targeted mutation 2.1, Takeshi SakuraiP518, QRFP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site2Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
icrephage P1 codon improved Cre (iCre)2icre

Information2

Donor DNABactriophage P1 Cre recombinase, SV40 poly A, Saccharomyces cerevisiae FRT site, mouse Qrfp genomic DNA
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nature. 2020 Jul;583(7814):109-114. doi: 10.1038/s41586-020-2163-6.
DepositorTakeshi Sakurai (University of Tsukuba)
Strain Statusan icon for Live miceLive mice
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Live mouse (1 to 3 months)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data