Strain Data Sheet

RBRC11132

Strain Information

Image
BRC No.RBRC11132
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Myb<tm1.1(T572A)Husm>
Former Common namec-Myb TA マウス
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeveloped by Kyoko Kitagawa, Hamamatsu University School of Medicine in 2020. Mice were generated using E14 ES cells derived from 129P2/OlaHsd. Mice were further backcrossed to C57BL/6J for 7 generations. C57BL/6J congenic line.
Strain descriptionMyb mutant harboring a point mutation (Thr572Ala).
Colony maintenance
References
Substitution of Thr572 to Ala in mouse c-Myb attenuates progression of early erythroid differentiation.
Kitagawa K, Uchida C, Horiguchi R, Ohhata T, Sakai S, Niida H, Yasumoto S, Handa Y, Suzuki M, Hashimoto M, Tazawa T, Yokochi Y, Tsuji M, Kitagawa M
Sci. Rep., 10(1):14381. doi: 10.1038/s41598-020-71267-5 (2020). 32873855

Fbw7 promotes ubiquitin-dependent degradation of c-Myb: involvement of GSK3-mediated phosphorylation of Thr-572 in mouse c-Myb.
Kitagawa K, Hiramatsu Y, Uchida C, Isobe T, Hattori T, Oda T, Shibata K, Nakamura S, Kikuchi A, Kitagawa M
Oncogene, 28(25):2393-405 (2009). 19421138

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Mybmyeloblastosis oncogene10Mybtargeted mutation 1.1, Chiharu Uchida
loxPphage P1 loxP10loxP

Phenotype

Annotation by Mammalian phenotyhpe ontology
Detailed phenotype data

Ordering Information

Donor DNAphage P1 loxP site, mouse c-Myb genomic DNA
Research applicationCre/loxP system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Sci. Rep., 10(1):14381. doi: 10.1038/s41598-020-71267-5 (2020).
DepositorChiharu Uchida (Hamamatsu University School of Medicine)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data