Strain Data Sheet

RBRC11130

Information1

Image
BRC No.RBRC11130
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6N-Tubb3<tm1.1(Cre)Ksak>
Former Common nameTubb3-Cre
H-2 Haplotype
ES Cell lineRENKA [C57BL/6NCrlCrlj]
Background strain
Appearance
Strain developmentDeposited by Kenji Sakimura, Niigata University in 2020. Mice were generated using RENKA ES cells derived from C57BL/6N. C57BL/6N genetic background.
Strain descriptionCre/IRES/EGFP knockin mice targeted at the Tubb3 gene locus.
Colony maintenance
ReferencesCell Rep., 36(4):109427(2021). 34320360

Health Report

Examination Date / Room / Rack2023/09/13Room:3-5Rack:B
2023/09/11Room:3-5Rack:B
2023/06/13Room:3-5Rack:B
2023/03/13Room:3-5Rack:B
2022/12/12Room:3-5Rack:B
2022/09/12Room:3-5Rack:B
2022/06/13Room:3-5Rack:B
2022/03/14Room:3-5Rack:B

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Tubb3tubulin, beta 3 class III8Tubb3

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
EGFPGreen Fluorescent Protein (Aequorea victoria)8EGFP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site8Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Iresinternal ribosomal entry site (EMCV)8

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
crePhage P1 Cre recombinase8cre

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
nlsSV40 large T antigen nuclear localization signal (NLS)8nls

Information2

Donor DNASV40 large T antigen nuclear localization signal (NLS), Bacteriophage P1 Cre recombinase, EMCV internal ribosomal entry site (IRES), Aequorea victoria EGFP cDNA, Saccharomyces cerevisiae Frt site, mouse Tubb3 genomic DNA
Research applicationCre/loxP system
FLP/frt system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Cell Rep., 36(4):109427(2021).The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research.
DepositorKenji Sakimura (Niigata University)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Live mouse (3 to 6 months)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data