Strain Data Sheet

RBRC11128

Information1

Image
BRC No.RBRC11128
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6;Cg-Tg(Eqtn-EGFP)1Ktos
Former Common nameEqtn<+/+>-Tg(Eqtn-EGFP)
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Kiyotaka Toshimori, Chiba university in 2020. Mice were generated using B6D2F1. Mice were further backcrossed to C57BL/6J for 3 generations. Mixed genetic background.
Strain descriptionTransgenic mice expressing EGFP under the control of Eqtn promoter.
Colony maintenance
ReferencesCell Tissue Res., 352(3):739-50 (2013). doi: 10.1007/s00441-013-1605-y. 23564009

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)UNEGFP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Eqtnequatorin, sperm acrosome associatedUNEqtnmouse Clgn promoter

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 poly AUN

Information2

Donor DNAmouse calmegin promoter, mouse Eqtn cDNA, Aequorea victoria EGFP cDNA, SV40 poly A
Research applicationFluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Cell Tissue Res. 2013 Jun;352(3):739-50. doi: 10.1007/s00441-013-1605-y.For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
DepositorKiyotaka Toshimori (Chiba University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)
  4. GFP Transfer License (Japanese / English)
    Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.

Genotyping protocol -PCR-

BRC mice in Publications

No Data