Strain Data Sheet

RBRC11127

Strain Information

Image
BRC No.RBRC11127
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129S2-Eqtn<tm1Ktos>
Former Common nameEqtn-KO, Eqtn<-/->
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain
Appearance
Strain developmentDeposited by Kiyotaka Toshimori, Chiba university in 2020. Mice were generated using R1 ES cells derived from 129. Mice were further backcrossed to C57BL/6J for 5 generations. Male homozygous mutants exhibit decreased fertility.
Strain descriptionEqtn knockout mice. Pgk-neo fragment replaced Exon 1 to 5 of the Eqtn gene.
Colony maintenance
References
Deletion of Eqtn in mice reduces male fertility and sperm-egg adhesion.
Ito C, Yamatoya K, Yoshida K, Fujimura L, Sugiyama H, Suganami A, Tamura Y, Hatano M, Miyado K, Toshimori K
Reproduction, 156(6):579-590 (2018). 30328350

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Eqtnequatorin, sperm acrosome associated4Eqtntargeted mutation 1, Kiyotaka Toshimori
loxPphage P1 loxP4loxP
loxPphage P1 loxP4loxP
neoneomycin resistance gene (E. coli)4mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
Detailed phenotype data

Ordering Information

Donor DNAP1 phage loxP sites, mouse Phosphoglycerate kinase promoter (PGK promoter) and poly A signal, E coli Neomycin, mouse Eqtn genomic DNA
Research applicationCre/loxP system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Reproduction, 156(6):579-590 (2018).For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
DepositorKiyotaka Toshimori (Chiba University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data