Strain Data Sheet

RBRC11120

Strain Information

Image
BRC No.RBRC11120
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameA/J-12<SM> Iah1<em1Kobam>
Former Common nameA/J-12<SM>-Iah1 knockout
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Misato Kobayashi, Nagoya University in 2020. Mice were generated using A/J-12<SM> with the CRISPR/Cas9.
Strain descriptionIah1 mutant A/J-12<SM> mice deleting 53 bp in exon 1 of the Iah1 gene.
Colony maintenance
References
Detection of differentially expressed candidate genes for a fatty liver QTL on mouse chromosome 12.
Kobayashi M, Suzuki M, Ohno T, Tsuzuki K, Taguchi C, Tateishi S, Kawada T, Kim Y I, Murai A, Horio F
BMC Genet., 17(1):73 (2016). 27266874

Ablation of Iah1, a candidate gene for diet-induced fatty liver, does not affect liver lipid accumulation in mice.
Masuya T, Suzuki M, Tsujimura J, Kanamori S, Miyasaka Y, Ohno T, Murai A, Horio F, Kobayashi M
PLoS One, 15(5):e0233087 (2020). 32407372

Searching for genetic factors of fatty liver in SMXA-5 mice by quantitative trait loci analysis under a high-fat diet.
Kumazawa M, Kobayashi M, Io F, Kawai T, Nishimura M, Ohno T, Horio F
J. Lipid Res., 48(9):2039-2046 (2007). 17595448

Genetic dissection of the fatty liver QTL Fl1sa by using congenic mice and identification of candidate genes in the liver and epididymal fat.
Suzuki M, Kobayashi M, Ohno T, Kanamori S, Tateishi S, Murai A, Horio F
BMC Genet., 17(1):145 (2016). 27855657

Health Report

Examination Date / Room / Rack2023/09/28Room:4-BRack:L
2023/08/28Room:4-BRack:L
2023/05/29Room:4-BRack:L
2023/02/27Room:4-BRack:L
2022/11/29Room:4-BRack:L

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Iah1isoamyl acetate-hydrolyzing esterase 1 homolog12Iah1endonuclease-mediated mutation 1, Fumihiko Horio

Phenotype

Annotation by Mammalian phenotyhpe ontology
Detailed phenotype data

Ordering Information

Donor DNAmouse chemically synthesized crRNA, mouse chemically synthesized tracrRNA
Research application
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. PLoS One, 15(5):e0233087 (2020). If the RECIPIENT desires to use the BIOLOGICAL RESOURCE for the purpose of For-profit Use of BIOLOGICAL RESOURCE, the RECIPIENT shall reach an agreement with the DEPOSITOR on terms and conditions of use of BIOLOGICAL RESOURCE and shall obtain a prior written consent from the DEPOSITOR.
DepositorFumihiko Horio (Nagoya University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data