Strain Data Sheet

RBRC11120

Information1

Image
BRC No.RBRC11120
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameA/J-12<SM> Iah1<em1Kobam>
Former Common nameA/J-12<SM>-Iah1 knockout
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Misato Kobayashi, Nagoya University in 2020. Mice were generated using A/J-12<SM> with the CRISPR/Cas9.
Strain descriptionIah1 mutant A/J-12<SM> mice deleting 53 bp in exon 1 of the Iah1 gene.
Colony maintenance
ReferencesJ. Lipid Res., 48(9):2039-2046 (2007). 17595448
BMC Genet., 17(1):145 (2016). 27855657
PLoS One, 15(5):e0233087 (2020). 32407372
BMC Genet., 17(1):73 (2016). 27266874

Health Report

Examination Date / Room / Rack2022/08/29Room:4-BRack:L
2022/07/25Room:4-BRack:L

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Iah1isoamyl acetate-hydrolyzing esterase 1 homolog12Iah1<em1Kobam>

Information2

Donor DNAmouse chemically synthesized crRNA, mouse chemically synthesized tracrRNA
Research application
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. PLoS One, 15(5):e0233087 (2020). If the RECIPIENT desires to use the BIOLOGICAL RESOURCE for the purpose of For-profit Use of BIOLOGICAL RESOURCE, the RECIPIENT shall reach an agreement with the DEPOSITOR on terms and conditions of use of BIOLOGICAL RESOURCE and shall obtain a prior written consent from the DEPOSITOR.
DepositorFumihiko Horio (Nagoya University)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data