Strain Data Sheet
RBRC11118
Strain Information | |
|---|---|
| Image | |
| BRC No. | RBRC11118 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | B6-Tlx1<tm1.1(cre/ERT2/ires2/Venus)Rygo> |
| Former Common name | Tlx1<CreER-Venus> |
| H-2 Haplotype | |
| ES Cell line | EGR-101 [C57BL/6N] |
| Background strain | |
| Appearance | |
| Strain development | Deposited by Ryo Goitsuka, Tokyo, Tokyo University of Science, in 2020. Mice were generated using ES cell line, EGR-101. C57BL/6J genetic background. |
| Strain description | Knock in mice expression Cre/ERT2 and Venus under the control of Tlx1 promoter. |
| Colony maintenance | |
| References | Generation of a Tlx1(CreER-Venus) knock-in mouse strain for the study of spleen development. Ryo Nakahara, Yasuhiro Kawai, Akihisa Oda, Miyuki Nishimura, Akikazu Murakami, Takachika Azuma, Tomonori Kaifu, Ryo Goitsuka Genesis, 52(11) 916-23 (2014). 25283275Niche-induced extramedullary hematopoiesis in the spleen is regulated by the transcription factor Tlx1. Oda A, Tezuka T, Ueno Y, Hosoda S, Amemiya Y, Notsu C, Kasahara T, Nishiyama C, Goitsuka R Sci. Rep., 8, 1-16 (2018). 29844356Transcription factor Tlx1 marks a subset of lymphoid tissue organizer-like mesenchymal progenitor cells in the neonatal spleen. Yuta Ueno, Keiko Fujisaki, Shoko Hosoda, Yusuke Amemiya, Shogo Okazaki, Chihiro Notsu, Chiharu Nishiyama, Yo Mabuchi, Yumi Matsuzaki, Akihisa Oda, Ryo Goitsuka Sci. Rep., 9, 20408 (2019). 31892733 |
Health Report | |
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| Examination Date / Room / Rack | 2024/05/20Room:4-ARack:L 2024/02/19Room:4-ARack:L 2023/11/20Room:4-ARack:L |
Gene | |||||||
|---|---|---|---|---|---|---|---|
| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| ERT2 | estrogen receptor (ER), human ER with the triple mutation G400V/M543A/L544A) | 19 | ERT2 | ||||
| Frt | yeast FRT (flippase recombination target) site | 19 | Frt | ||||
| Ires | internal ribosomal entry site (EMCV) | 19 | |||||
| Tlx1 | T cell leukemia, homeobox 1 | 19 | Tlx1 | targeted mutation 1.1, Ryo Goitsuka | |||
| Venus | Yellow Fluorescent Protein (Aequorea victoria) | 19 | Venus | ||||
| cre | Phage P1 Cre recombinase | 19 | cre | ||||
Phenotype | |
|---|---|
| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
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| Donor DNA | Phage P1 Cre recombinase, human estrogen receptor (ER) cDNA, EMCV IRES2, jellyfish Yellow Fluorescent Protein (Aequorea victoria) cDNA, yeast FRT (flippase recombination target) site |
| Research application | Cre/loxP system FLP/frt system Fluorescent Proteins/lacZ System |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genesis, 52(11) 916-23 (2014).In publishing the research results obtained by use of the BIOLOGICAL RESOURCE containing Venus, an acknowledgment to Dr. Atsushi Miyawaki and a citation of the following literature(s) designated by the DEPOSITOR are requested. Nat. Biotechnol., 20, 87-90 (2002). |
| Depositor | Ryo Goitsuka (Tokyo University of Science) |
| Strain Status |  Frozen embryos Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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| No Data |