Strain Data Sheet

RBRC11118

Information1

Image
BRC No.RBRC11118
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6-Tlx1<tm1.1(cre/ERT2/ires2/Venus)Rygo>
Former Common nameTlx1<CreER-Venus>
H-2 Haplotype
ES Cell lineEGR-101 [C57BL/6N]
Background strain
Appearance
Strain developmentDeposited by Ryo Goitsuka, Tokyo, Tokyo University of Science, in 2020. Mice were generated using ES cell line, EGR-101. C57BL/6J genetic background.
Strain descriptionKnock in mice expression Cre/ERT2 and Venus under the control of Tlx1 promoter.
Colony maintenance
ReferencesSci. Rep., 9, 20408 (2019). 31892733
Genesis, 52(11) 916-23 (2014). 25283275
Sci. Rep., 8, 1-16 (2018). 29844356

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Tlx1T cell leukemia, homeobox 119Tlx1<tm1.1(cre/ERT2/ires2/Venus)Rygo>targeted mutation 1.1, Ryo Goitsuka

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
ERT2estrogen receptor (ER), human ER with the triple mutation G400V/M543A/L544A)19ERT2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site19Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Iresinternal ribosomal entry site (EMCV)19

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
VenusYellow Fluorescent Protein (Aequorea victoria)19Venus

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
crePhage P1 Cre recombinase19cre

Information2

Donor DNAPhage P1 Cre recombinase, human estrogen receptor (ER) cDNA, EMCV IRES2, jellyfish Yellow Fluorescent Protein (Aequorea victoria) cDNA, yeast FRT (flippase recombination target) site
Research applicationCre/loxP system
FLP/frt system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genesis. 2014 Nov;52(11):916-23.
DepositorRyo Goitsuka (Tokyo University of Science)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Live mouse (3 to 6 months)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEVELOPER of the Venus using Approval form (Form V (English)) (Lab Contact: Laboratory for Cell Function Dynamics, RIKEN CBS: mta-cfds@ml.riken.jp).
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data