Strain Data Sheet
RBRC11116
Strain Information | |
|---|---|
| Image | |
| BRC No. | RBRC11116 |
| Type | Transgene |
| Species | Mus musculus |
| Strain name | B6.129P2-Tg(Ddx4-RFP)#15Ymat |
| Former Common name | Vasa-RFP |
| H-2 Haplotype | |
| ES Cell line | E14.1 [129P2/OlaHsd] |
| Background strain | |
| Appearance | |
| Strain development | Developed by Yasuhisa Matsui, Tohoku University, in 2006. Mice were generated using E14-1 ES cells derived from 129P2/OlaHsd and further backcrossed to C57BL/6 for more than 10 generations. |
| Strain description | BAC transgenic mice expressing RFP under the control of Ddx4 promoter. |
| Colony maintenance | |
| References | Induction of primordial germ cells from mouse induced pluripotent stem cells derived from adult hepatocytes. Imamura M, Aoi T, Tokumasu A, Mise N, Abe K, Yamanaka S, Noce T Mol. Reprod. Dev., 77(9):802-11 (2010). 20722049Control of epigenomic landscape and development of fetal male germ cells through L-serine metabolism. Yohei Hayashi, Jintaro Kaneko, Yumi Ito-Matsuoka, Asuka Takehara, Mayuka Funakoshi, So Maezawa, Kenjiro Shirane, Shigeki Furuya, Yasuhisa Matsui ISCIENCE, 9;27(9):110702 (2024). 39262797 |
Health Report | |
|---|---|
| Examination Date / Room / Rack | 2025/07/28Room:4-ARack:ESentinel mouse program 2025/05/19Room:4-ARack:ESentinel mouse program 2025/02/17Room:4-ARack:ESentinel mouse program 2024/11/18Room:4-ARack:ESentinel mouse program 2024/08/19Room:4-ARack:ESentinel mouse program |
Gene | |||||||
|---|---|---|---|---|---|---|---|
| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| Frt | yeast FRT (flippase recombination target) site | UN | Frt | ||||
| GH | Growth hormone polyA (Bovine) | UN | GH | ||||
| RFP | Red Fluorescent Protein (Discosoma sp.) | UN | RFP | mouse Ddx4 promoter | |||
| loxP | phage P1 loxP | UN | loxP | ||||
| neo | neomycin resistance gene (E. coli) | UN | |||||
Phenotype | |
|---|---|
| Phenotype annotation from literatures by Mammalian phenotype ontology | |
| Detailed phenotype data | |
Ordering Information | |
|---|---|
| Donor DNA | mouse Ddx4 (Vasa) genomic DNA, Discosoma sp. mRFP1 cDNA, E. coli Neomycin resistance gene, bovine growth hormone polyadenylation site, phage P1 loxP site (BAC vector), yeast FRT (flippase recombination target) sites |
| Research application | Cre/loxP system FLP/frt system Fluorescent Proteins/lacZ System |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Mol. Reprod. Dev., 77(9):802-11 (2010). |
| Depositor | Yasuhisa Matsui (Tohoku University) |
| Strain Status |  Frozen embryos Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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| No Data |