Strain Data Sheet

RBRC11116

Strain Information

Image
BRC No.RBRC11116
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6.129P2-Tg(Ddx4-RFP)#15Ymat
Former Common nameVasa-RFP
H-2 Haplotype
ES Cell lineE14.1 [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeveloped by Yasuhisa Matsui, Tohoku University, in 2006. Mice were generated using E14-1 ES cells derived from 129P2/OlaHsd and further backcrossed to C57BL/6 for more than 10 generations.
Strain descriptionBAC transgenic mice expressing RFP under the control of Ddx4 promoter.
Colony maintenance
References
Induction of primordial germ cells from mouse induced pluripotent stem cells derived from adult hepatocytes.
Imamura M, Aoi T, Tokumasu A, Mise N, Abe K, Yamanaka S, Noce T
Mol. Reprod. Dev., 77(9):802-11 (2010). 20722049

ISCIENCE, 9;27(9):110702 (2024). 39262797

Health Report

Examination Date / Room / Rack2024/08/19Room:4-ARack:E

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frtyeast FRT (flippase recombination target) siteUNFrt
GHGrowth hormone polyA (Bovine)UNGH
RFPRed Fluorescent Protein (Discosoma sp.)UNRFPmouse Ddx4 promoter
loxPphage P1 loxPUNloxP
neoneomycin resistance gene (E. coli)UN

Phenotype

Annotation by Mammalian phenotyhpe ontology
Detailed phenotype data

Ordering Information

Donor DNAmouse Ddx4 (Vasa) genomic DNA, Discosoma sp. mRFP1 cDNA, E. coli Neomycin resistance gene, bovine growth hormone polyadenylation site, phage P1 loxP site (BAC vector), yeast FRT (flippase recombination target) sites
Research applicationCre/loxP system
FLP/frt system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Mol. Reprod. Dev., 77(9):802-11 (2010).
DepositorYasuhisa Matsui (Tohoku University)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityLive mouse (1 to 3 months)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: FP license of TBUSA
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data