Strain Data Sheet

RBRC11115

Strain Information

Image
BRC No.RBRC11115
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129P2-Rest<tm1Ymat>
Former Common nameRest-KO
H-2 Haplotype
ES Cell lineE14.1 [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeveloped by Yasuhisa Matsui, Tohoku University, in 2012. Mice were generated using E14-1 ES cells derived from 129P2/OlaHsd. Mixed genetic background with C57BL/6.
Strain descriptionRest knockout mice. Exon 1 was replaced with a pgk-neo cassette in the inverse orientation via homologous recombination.
Colony maintenance
References
REST and its downstream molecule Mek5 regulate survival of primordial germ cells.
Okamura D, Mochizuki K, Taniguchi H, Tokitake Y, Ikeda M, Yamada Y, Tournier C, Yamaguchi S, Tada T, Scholer H R, Matsui Y
Dev. Biol., 372(2):190-202 (2012). 23022299

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
RestRE1-silencing transcription factor5Resttargeted mutation 1, Yasuhisa Matsui
  • autosomal dominant nonsyndromic hearing loss 27(MedGEN)

  • fibromatosis, gingival, 5(MedGEN)

  • Wilms tumor 6(MedGEN)
    • loxPphage P1 loxP5loxP
  • autosomal dominant nonsyndromic hearing loss 27(MedGEN)

  • fibromatosis, gingival, 5(MedGEN)

  • Wilms tumor 6(MedGEN)
    • loxPphage P1 loxP5loxP
  • autosomal dominant nonsyndromic hearing loss 27(MedGEN)

  • fibromatosis, gingival, 5(MedGEN)

  • Wilms tumor 6(MedGEN)
    • neoneomycin resistance gene (E. coli)5mouse phosphoglycerate kinase promoter (PGK promoter)
  • autosomal dominant nonsyndromic hearing loss 27(MedGEN)

  • fibromatosis, gingival, 5(MedGEN)

  • Wilms tumor 6(MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
    Detailed phenotype data

    Ordering Information

    Donor DNAphage P1 loxP sites, mouse phosphoglycerate kinase promo (Pgk1 promoter), E. coli neomycin resistant gene, polyA付加配列
    Research applicationCre/loxP system
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Dev Biol. 2012 Dec 15;372(2):190-202.
    DepositorYasuhisa Matsui (Tohoku University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    Strain AvailabilityRecovery and QC required prior to distribution
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    BRC mice in Publications

    No Data