Strain Data Sheet

RBRC11113

Strain Information

Image
BRC No.RBRC11113
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameD1.129P2-Cxcr4<tm1Yiw>
Former Common nameCXCR4 KO/DBA
H-2 Haplotype
ES Cell lineE14.1 [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeposited by Yoichiro Iwakura, Tokyo University of Science, in 2020. Mice were generated using E14.1 ES cells derived from 129P2/OlaHsd. Mice were further backcrossed to DBA/1 for 7 generations. DBA/1 congenic strain.
Strain descriptionExon 2 was floxed and a neomycin resistant cassette was inserted 5' to the second loxP site within intron 2.
Colony maintenance
References
CXC chemokine receptor 4 expressed in T cells plays an important role in the development of collagen-induced arthritis.
Chung S H, Seki K, Choi B I, Kimura K B, Ito A, Fujikado N, Saijo S, Iwakura Y
Arthritis Res Ther., 12(5):R188 (2010). 20939892

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Cxcr4
MGI:109563		C-X-C motif chemokine receptor 41Cxcr4<tm1Yiw>
MGI:4836915		targeted mutation 1, Yoichiro Iwakura
  • WHIM syndrome 1(MedGEN)

  • WHIM syndrome(DisGeNET)
    • GH Growth hormone polyA (Bovine)1GH
    loxP phage P1 loxP1loxP
    loxP phage P1 loxP1loxP
    neo neomycin resistance gene (E. coli)1 mouse phosphoglycerate kinase promoter (PGK promoter)

    Phenotype

    Phenotype annotation from literatures by Mammalian phenotype ontology
    Detailed phenotype data

    Ordering Information

    Donor DNAP1 Phage loxP sites, mouse Phosphoglycerate kinase (PGK) promoter, E.coli Neomycin resistant gene, Bovine growth hormone polyadenylation site, mouse Cxcr4 genomic DNA
    Research applicationCre/loxP system
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
    DepositorYoichiro Iwakura (Tokyo University of Science)
    Strain Statusan icon for Frozen embryosFrozen embryos
    Strain AvailabilityRecovery and QC required prior to distribution
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    BRC mice in Publications

    No Data