Strain Data Sheet

RBRC11113

Information1

Image
BRC No.RBRC11113
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameD1.129P2-Cxcr4<tm1Yiw>
Former Common nameCXCR4 KO/DBA
H-2 Haplotype
ES Cell lineE14.1 [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeposited by Yoichiro Iwakura, Tokyo University of Science, in 2020. Mice were generated using E14.1 ES cells derived from 129P2/OlaHsd. Mice were further backcrossed to DBA/1 for 7 generations. DBA/1 congenic strain.
Strain descriptionExon 2 was floxed and a neomycin resistant cassette was inserted 5' to the second loxP site within intron 2.
Colony maintenance
ReferencesArthritis Res Ther., 12(5):R188 (2010). 20939892

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Cxcr4chemokine (C-X-C motif) receptor 41Cxcr4<tm1Yiw>targeted mutation 1, Yoichiro Iwakura

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GHGrowth hormone polyA (Bovine)1GH

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP1loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP1loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)1mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAP1 Phage loxP sites, mouse Phosphoglycerate kinase (PGK) promoter, E.coli Neomycin resistant gene, Bovine growth hormone polyadenylation site, mouse Cxcr4 genomic DNA
Research applicationCre/loxP system
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
DepositorYoichiro Iwakura (Tokyo University of Science)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data