Strain Data Sheet
RBRC11102
Strain Information | |
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| Image | |
| BRC No. | RBRC11102 |
| Type | Targeted Mutation Congenic |
| Species | Mus musculus |
| Strain name | B6.Cg-Nipal4<tm1Akih> |
| Former Common name | Nipal4 KO |
| H-2 Haplotype | |
| ES Cell line | TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1] |
| Background strain | |
| Appearance | |
| Strain development | Deposited by Akio Kihara, Hokkaido University, in 2020. Mice were generated using TT2 (ES cell) derived from (C57BL/6NCrlj x CBA/JNCrlj)F1. Mice were further backcrossed to C57BL/6J for 11 generations. |
| Strain description | Exon 5 was replaced with an FRT-flanked neomycin resistance cassette. RT-PCR analysis confirmed absence of mRNA expression in the epidermis of homozygous mutant mice. |
| Colony maintenance | |
| References | Decreased Skin Barrier Lipid Acylceramide and Differentiation-Dependent Gene Expression in Ichthyosis Gene Nipal4-Knockout Mice. Honda Y, Kitamura T, Naganuma T, Abe T, Ohno Y, Sassa T, Kihara A J. Invest. Dermatol., 138, 741-749 (2018). 29174370 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |||||||
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| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| SV40 polyA signal | 11 | ||||||
| Frt | yeast FRT (flippase recombination target) site | 11 | Frt | ||||
| Frt | yeast FRT (flippase recombination target) site | 11 | Frt | ||||
| Nipal4 | NIPA-like domain containing 4 | 11 | Nipal4 | targeted mutation 1, Akio Kihara | |||
| loxP | phage P1 loxP | 11 | loxP | ||||
| neo | neomycin resistance gene (E. coli) | 11 | mouse Phosphoglycerate kinase promoter (PGK promoter), synthetic bacterial EM7 promoter (T7 phage) | ||||
Phenotype | |
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| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
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| Donor DNA | Saccharomyces cerevisiae FRT sites, synthetic bacterial EM7 promoter (T7 phage), Mus musculus Phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistant gene, SV40 PolyA signal, Bacteriophage P1 LoxP site, mouse Nipal4 genomic DNA |
| Research application | Cre/loxP system FLP/frt system |
| Specific Term and Conditions | In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. |
| Depositor | Akio Kihara (Hokkaido University) |
| Strain Status |  Frozen embryos Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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| No Data |