Strain Data Sheet

RBRC11102

Information1

Image
BRC No.RBRC11102
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Nipal4<tm1Akih>
Former Common nameNipal4 KO
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeposited by Akio Kihara, Hokkaido University, in 2020. Mice were generated using TT2 (ES cell) derived from (C57BL/6NCrlj x CBA/JNCrlj)F1. Mice were further backcrossed to C57BL/6J for 11 generations.
Strain descriptionExon 5 was replaced with an FRT-flanked neomycin resistance cassette. RT-PCR analysis confirmed absence of mRNA expression in the epidermis of homozygous mutant mice.
Colony maintenance
ReferencesJ. Invest. Dermatol., 138, 741-749 (2018). 29174370

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Nipal4NIPA-like domain containing 411Nipal4<tm1Akih>targeted mutation 1, Akio Kihara

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site11Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site11Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP11loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)11mouse Phosphoglycerate kinase promoter (PGK promoter), E. coli EM7 promoter

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 PolyA signal11

Information2

Donor DNASaccharomyces cerevisiae FRT sites, E. coli EM7 promoter, Mus musculus Phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistant gene, SV40 PolyA signal, Bacteriophage P1 LoxP site, mouse Nipal4 genomic DNA
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsIn publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
DepositorAkio Kihara (Hokkaido University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data