Strain Data Sheet

RBRC11100

Information1

Image
BRC No.RBRC11100
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129-Elovl1<tm1.1Akih>
Former Common nameElovl1 KO
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeposited by Akio Kihara, Hokkaido University, in 2020. Mice were generated using E14 (ES cell) derived from 129P2/OlaHsd. Mice were further backcrossed to C57BL/6J for more than 21 generations.
Strain descriptionElovl1 knockout mice. A loxP site was inserted upstream of exon 2. An FRT-flanked neomycin resistance cassette with a 5' loxP site was inserted downstream of exon 8. Cre-mediated recombination removed exons 2 through 8 and left the FRT-flanked neomycin resistance cassette intact.
Colony maintenance
ReferencesMol. Cell. Biol., 33, 2787-2796 (2013). 23689133

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Elovl1elongation of very long chain fatty acids (FEN1/Elo2, SUR4/Elo3, yeast)-like 14Elovl1<tm1.1Akih>targeted mutation 1.1, Akio Kihara

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site4Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site4Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP4loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)4mouse Phosphoglycerate kinase promoter (PGK promoter), E. coli EM7 promoter

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 PolyA4

Information2

Donor DNABacteriophage P1 loxP site, Saccharomyces cerevisiae FRT sites, E. coli EM7 promoter, Mouse Phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistant gene, SV40 PolyA signal, mouse Elovl1 genomic DNA
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsIn publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
DepositorAkio Kihara (Hokkaido University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data