Strain Data Sheet

RBRC11100

Strain Information

Image
BRC No.RBRC11100
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129-Elovl1<tm1.1Akih>
Former Common nameElovl1 KO
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeposited by Akio Kihara, Hokkaido University, in 2020. Mice were generated using E14 (ES cell) derived from 129P2/OlaHsd. Mice were further backcrossed to C57BL/6J for more than 21 generations.
Strain descriptionElovl1 knockout mice. A loxP site was inserted upstream of exon 2. An FRT-flanked neomycin resistance cassette with a 5' loxP site was inserted downstream of exon 8. Cre-mediated recombination removed exons 2 through 8 and left the FRT-flanked neomycin resistance cassette intact.
Colony maintenance
References
Impaired epidermal permeability barrier in mice lacking elovl1, the gene responsible for very-long-chain fatty acid production.
Sassa T, Ohno Y, Suzuki S, Nomura T, Nishioka C, Kashiwagi T, Hirayama T, Akiyama M, Taguchi R, Shimizu H, Itohara S, Kihara A
Mol. Cell. Biol., 33, 2787-2796 (2013). 23689133

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
SV40 polyA4
  • ichthyotic keratoderma, spasticity, hypomyelination, and dysmorphic facial features(MedGEN)
    • Elovl1ELOVL fatty acid elongase 14Elovl1targeted mutation 1.1, Akio Kihara
  • ichthyotic keratoderma, spasticity, hypomyelination, and dysmorphic facial features(MedGEN)
    • Frtyeast FRT (flippase recombination target) site4Frt
  • ichthyotic keratoderma, spasticity, hypomyelination, and dysmorphic facial features(MedGEN)
    • Frtyeast FRT (flippase recombination target) site4Frt
  • ichthyotic keratoderma, spasticity, hypomyelination, and dysmorphic facial features(MedGEN)
    • loxPphage P1 loxP4loxP
  • ichthyotic keratoderma, spasticity, hypomyelination, and dysmorphic facial features(MedGEN)
    • neoneomycin resistance gene (E. coli)4mouse Phosphoglycerate kinase promoter (PGK promoter), synthetic bacterial EM7 promoter (T7 phage)
  • ichthyotic keratoderma, spasticity, hypomyelination, and dysmorphic facial features(MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
    Detailed phenotype data

    Ordering Information

    Donor DNABacteriophage P1 loxP site, Saccharomyces cerevisiae FRT sites, synthetic bacterial EM7 promoter (T7 phage), Mouse Phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistant gene, SV40 PolyA signal, mouse Elovl1 genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Specific Term and ConditionsIn publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
    DepositorAkio Kihara (Hokkaido University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data