Strain Data Sheet

RBRC11098

Strain Information

Image
BRC No.RBRC11098
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Scx<tm1.1(EGFP)Skom>
Former Common nameScx-EGFP
H-2 Haplotype
ES Cell lineV6.5 [(C57BL/6J x 129S4/SvJae)F1]
Background strain
Appearance
Strain developmentDeposited by Shingo Komura, Gifu University in 2020. Mice were generated using v6.5 ES cells derived from (C57BL/6 x 129S4/SvJae)F1. Mice were further backcrossed to C57BL/6J for 6-7 generations. C57BL/6J congenic line.
Strain descriptionMice harboring IRES EGFP knock in at 3’ UTR of the Scx gene.
Colony maintenance
References
Induced pluripotent stem cell-derived tenocyte-like cells promote the regeneration of injured tendons in mice.
Komura S, Satake T, Goto A, Aoki H, Shibata H, Ito K, Hirakawa A, Yamada Y, Akiyama H
Sci. Rep., 10(1):3992 (2020). 32132649

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)15EGFP
HBB2rabbit beta-globin polyA15HBB2
IRESinternal ribosomal entry site (EMCV)15
Scxscleraxis scleraxis bHLH transcription factor15Scxtargeted mutation 1.1, Shingo Komura
roxBacteriophage D6 rox site15rox

Phenotype

Annotation by Mammalian phenotyhpe ontology
Detailed phenotype data

Ordering Information

Donor DNAEncephalomyocarditisvirus (EMCV) internal ribosomal entry site (ires), Aequorea Victoria EGFP cDNA, Rabbit beta globin pA, D6 Bacteriophage rox site, mouse Scx genomic DNA
Research applicationCell Biology Research
Cre/loxP system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Sci Rep. 2020 Mar 4;10(1):3992.The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research.
DepositorShingo Komura (Gifu University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data