Strain Data Sheet

RBRC11098

Information1

Image
BRC No.RBRC11098
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Scx<tm1.1(EGFP)Skom>
Former Common nameScx-EGFP
H-2 Haplotype
ES Cell lineV6.5 [(C57BL/6J x 129S4/SvJae)F1]
Background strain
Appearance
Strain developmentDeposited by Shingo Komura, Gifu University in 2020. Mice were generated using v6.5 ES cells derived from (C57BL/6 x 129S4/SvJae)F1. Mice were further backcrossed to C57BL/6J for 6-7 generations. C57BL/6J congenic line.
Strain descriptionMice harboring IRES EGFP knock in at 3’ UTR of the Scx gene.
Colony maintenance
ReferencesSci. Rep., 10(1):3992 (2020). 32132649

Health Report

Examination Date / Room / Rack2022/02/14Room:3-CRack:K
2021/11/15Room:3-CRack:K
2021/08/16Room:3-CRack:K
2021/07/18Room:3-CRack:K

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Scxscleraxis15Scx<tm1.1(EGFP)Skom>

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)15EGFP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
HBB2rabbit beta-globin polyA15HBB2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
IRESinternal ribosomal entry site (EMCV)15

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
roxD6 Bacteriophage rox site15rox

Information2

Donor DNAEncephalomyocarditisvirus (EMCV) internal ribosomal entry site (ires), Aequorea Victoria EGFP cDNA, Rabbit beta globin pA, D6 Bacteriophage rox site, mouse Scx genomic DNA
Research applicationCell Biology Research
Cre/loxP system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Sci Rep. 2020 Mar 4;10(1):3992.The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research.
DepositorShingo Komura (Gifu University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-
Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)
  4. GFP Transfer License (Japanese / English)
    Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.

BRC mice in Publications

No Data