Strain Data Sheet

RBRC11096

Information1

Image
BRC No.RBRC11096
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129-Myh9<tm1(R702C)Dash>
Former Common nameR702C Neo
H-2 Haplotype
ES Cell lineD3 [129S2/SvPas]
Background strain
Appearance
Strain developmentDeposited by Tadashi Matushita in 2020. Mice were generated using D3 ES cells derived from D3(129Sv). Mice were backcrossed to C57BL/6 over 3 years by the depositor.
Strain descriptionCre-dependent conditional mutant expressing a mutated Myh9 (R702C). A floxed pgk-neo was inserted between exon 14 and 15.
Colony maintenance
ReferencesPLoS One, 8(8):e71187 (2013). 23976996

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Myh9myosin, heavy polypeptide 9, non-muscle15Myh9<tm1(R702C)Dash>

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP15loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP15loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)15mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAマウス 129SVJ Myh9, 大腸菌 Neo, Bacteriphage P1 loxP, マウス 129SVJ PGK
Research applicationCre/loxP system
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. PLoS One, 8(8):e71187 (2013).The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of its use with the DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR.
DepositorTadashi Matsushita (Nagoya University)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data