Strain Data Sheet

RBRC11093

Strain Information

Image
BRC No.RBRC11093
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6-Tg(RP24-310A6/Luc)2MftymMfuku
Former Common nameBDNF-Luc
H-2 Haplotype
ES Cell lineRENKA [C57BL/6NCrlCrlj]
Background strain
Appearance
Strain developmentDeposited by Mamoru Fukuchi in 2020. C57BL/6 genetic background.
Strain descriptionBac transgenic mice expressing Luc2 under the control of the BDNF gene promoter. Luc2 was inserted at exon IX of the Bdnf gene.
Colony maintenance
References
Neuromodulatory effect of Galphas- or Galphaq-coupled G-protein-coupled receptor on NMDA receptor selectively activates the NMDA receptor/Ca2+/calcineurin/cAMP response element-binding protein-regulated transcriptional coactivator 1 pathway to effectively induce brain-derived neurotrophic factor expression in neurons.
Fukuchi M, Tabuchi A, Kuwana Y, Watanabe S, Inoue M, Takasaki I, Izumi H, Tanaka A, Inoue R, Mori H, Komatsu H, Takemori H, Okuno H, Bito H, Tsuda M
J. Neurosci., 35(14):5606-24 (2015). 25855176

Visualizing changes in brain-derived neurotrophic factor (BDNF) expression using bioluminescence imaging in living mice.
Fukuchi M, Izumi H, Mori H, Kiyama M, Otsuka S, Maki S, Maehata Y, Tabuchi A, Tsuda M
Sci. Rep., 7(1):4949 (2017). 28694523

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
luc Photinus pyralis LuciferaseUNluc

Phenotype

Annotation by Mammalian phenotyhpe ontology
Detailed phenotype data

Ordering Information

Donor DNAPhotinus pyralis Luciferase (Promega社:luc2), mouse BDNF genomic DNA (BAC clone RP24-310A6)
Research application
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Neurosci., 35(14):5606-24 (2015). Sci. Rep., 7(1):4949 (2017).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for institution fora not-for-profit research.
DepositorMamoru Fukuchi (Takasaki University of Health and Welfare)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data