Strain Data Sheet

RBRC11090

Strain Information

Image
BRC No.RBRC11090
TypeCRISPR/Cas9 (Transgene)Cartagena
SpeciesMus musculus
Strain nameC57BL6/J-Actb<em1(tetO-PA-cre)ttaka>
Former Common nameTRE-PA-Cre
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped and deposited by Takeshi Takarada, in 2020. Mice were generated using C57BL/6J with the CRISPR/Cas9. C57BL/6J genetic background.
Strain descriptionKnockin mice in which TRE-PA (photoactivatable)-Cre is placed downstream of exon 6 of the Actb.
Colony maintenance
ReferencesBiochem. Biophys. Res. Commun., 526(1):213-217 (2020). 32204914

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Actbactin, beta5Actb<em1(tetO-PA-cre)ttaka>endonuclease-mediated mutation 1, Takeshi Takarada

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
P2Aporcine teschovirus-1 2A peptide5P2AE. coli tetO (tetracycline operator), CMV promoter

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
crePhage P1 Cre recombinase5cre

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
nlsSV40 large T antigen nuclear localization signal (NLS)5nls

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyA5

Ordering Information

Donor DNACytomegalovirus minimum CMV promoter, E. coli TRE(Tetracycline response element;tetO operator sequence), phage P1 Cre recombinase, Neurospora crassa pMag/nMag, SV40 nls, P2A (porcine teschovirus-1 2A peptide), SV40 polyA, mouse Actb genomic DNA
Research applicationCre/loxP system
Tet system
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Biochem. Biophys. Res. Commun., 526(1):213-217 (2020).When the RECIPIENT obtain approval from the DEPOSITOR. the RECIPIENT must agreement on terms transfer and conditions of use of it with DEPOSITOR.
DepositorTakeshi Takarada (Okayama University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-
Mouse of the Month Mar 2022

BRC mice in Publications

No Data