Strain Data Sheet

RBRC11090

Strain Information

Image
BRC No.RBRC11090
TypeCRISPR/Cas9 (Transgene)Cartagena
SpeciesMus musculus
Strain nameC57BL6/J-Actb<em1(tetO-PA-cre)ttaka>
Former Common nameTRE-PA-Cre
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped and deposited by Takeshi Takarada, in 2020. Mice were generated using C57BL/6J with the CRISPR/Cas9. C57BL/6J genetic background.
Strain descriptionKnockin mice in which TRE-PA (photoactivatable)-Cre is placed downstream of exon 6 of the Actb.
Colony maintenance
References
Establishment of a tTA-dependent photoactivatable Cre recombinase knock-in mouse model for optogenetic genome engineering.
Takao T, Hiraoka Y, Kawabe K, Yamada D, Ming L, Tanaka K, Sato M, Takarada T
Biochem. Biophys. Res. Commun., 526(1):213-217 (2020). 32204914

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Actb
MGI:87904		actin, beta5Actb<em1(tetO-PA-cre)ttaka> endonuclease-mediated mutation 1, Takeshi Takarada
  • autosomal dominant nonsyndromic hearing loss 20(MedGEN)

  • Baraitser-Winter syndrome 1(MedGEN)

  • Baraitser-winter syndrome 2(MedGEN)
    • more 1 Diseases
  • developmental malformations-deafness-dystonia syndrome(MedGEN)
    • P2A porcine teschovirus-1 2A peptide5P2A E. coli tetO (tetracycline operator), CMV promoter
    cre Phage P1 Cre recombinase5cre
    nls SV40 large T antigen nuclear localization signal (NLS)5nls
    SV40 polyA5

    Phenotype

    Phenotype annotation from literatures by Mammalian phenotype ontology
    Detailed phenotype data

    Ordering Information

    Donor DNACytomegalovirus minimum CMV promoter, E. coli TRE(Tetracycline response element；tetO operator sequence), phage P1 Cre recombinase, Neurospora crassa pMag/nMag, SV40 nls, P2A (porcine teschovirus-1 2A peptide), SV40 polyA, mouse Actb genomic DNA
    Research applicationCre/loxP system
    Tet system
    Cre driver
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Biochem. Biophys. Res. Commun., 526(1):213-217 (2020).When the RECIPIENT obtain approval from the DEPOSITOR. the RECIPIENT must agreement on terms transfer and conditions of use of it with DEPOSITOR.
    DepositorTakeshi Takarada (Okayama University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-
    Mouse of the Month Mar 2022

    BRC mice in Publications

    No Data